Immunoelectron microscopic demonstration of prostatic acid phosphatase in human hyperplastic prostate.
Immunoelectron microscopic demonstration of prostatic acid phosphatase in human hyperplastic prostate.
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人增生性前列腺中前列腺酸性磷酸酶的免疫电子显微镜演示。
DOI：
10.1002/pros.2990070108
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Yam,LT
中科院分区：
文献类型：
--
作者：
Song,GX;Lin,CT;Wu,JY;Lam,KW;Li,CY;Yam,LT
对良性前列腺增生患者的前列腺组织进行了免疫电镜研究。使用抗纯化人前列腺酸性磷酸酶带2（HPAP-2）的兔IgG-过氧化物酶结合物进行研究。光镜下，前列腺柱状分泌上皮呈不同强度和分布的免疫组化染色，而基底细胞不染色。电镜下，分泌上皮细胞的高尔基体、分泌囊泡和空泡内常可见电子致密反应产物，核膜池和粗糙内质网内少见。有时可见分泌泡膜与质膜融合，分泌泡内容物排入细胞外。柱状上皮顶端微绒毛表面及腺泡内腔均可见反应产物。这些发现表明，HPAP-2可能在粗内质网中合成并运输到高尔基体，在那里它被浓缩并转移到分泌囊泡和液泡。HPAP-2最终通过胞吐作用（一种与其他分泌蛋白类似的分泌机制）被排出到细胞外空间。
Immunoelectron microscopic studies were done on prostatic tissues obtained from patients with benign hyperplasia. Rabbit IgG‐peroxidase conjugate against purified human prostatic acid phosphatase band 2 (HPAP‐2) was used for studies. Under the light microscope, the columnar secretory epithelia of prostatic glands showed different intensity and distribution of immunostaining whereas the basal cells were unstained. Under the electron microscope, the secretory epithelial cells often showed electron‐dense reaction product in the Golgi apparatus and secretory vesicles and vacuoles, and only sparingly in the cisternae of nuclear envelope and rough ER. Sometimes, fusion of secretory vacuolar membrane and plasma membrane and discharge of the vacuolar contents into the extracellular space were noted. The surfaces of microvilli at the apical portion of the columnar epithelia and the lumen of the glandular acini always showed reaction product. These findings suggest that HPAP‐2 may be synthesized in the rough ER and transported to the Golgi apparatus where it is concentrated and transferred to the secretory vesicles and vacuoles. HPAP‐2 is finally discharged into the extracellular spaces through exocytosis, a secretory mechanism similar to that of other secretory proteins.