ISOLATION OF X-RAY-INDUCIBLE TRANSCRIPTS FROM RADIORESISTANT HUMAN-MELANOMA CELLS
ISOLATION OF X-RAY-INDUCIBLE TRANSCRIPTS FROM RADIORESISTANT HUMAN-MELANOMA CELLS
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DOI：
10.1073/pnas.90.15.7200
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发表时间：
1993-08-01
影响因子：
11.1
通讯作者：
LEE, SW
中科院分区：
文献类型：
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作者：
BOOTHMAN, DA;MEYERS, M;LEE, SW
12个X射线诱导的成绩单（XIPS），差异表达8- 230倍，在X射线照射与未照射的抗辐射的人黑色素瘤（U1-Mel）细胞，分离的cDNA克隆（XIP 1至XIP 12）经过四轮的差异杂交。北方分析揭示了罕见的，中等的，和丰富的xip，大小范围从1.2到10 kb。所有转录本均瞬时表达，并由低剂量辐射诱导，但不受高剂量辐射（>600 cGy）诱导。三种转录物（xip 4、xip-7和xip-12）仅由电离辐射诱导，并且许多转录物（即，xip 1、-2、-3、-5、-6、-8、-9、-10和-11）也通过UV照射或佛波醇12-肉豆蔻酸酯13-乙酸酯诱导。热休克没有诱导任何xip，但它降低了xip 4，-7，-11和-12的基础水平。三个XIP cDNA克隆被鉴定为编码胸苷激酶、DT心肌黄酶和组织型纤溶酶原激活剂。其余9个cDNA克隆显示与已知基因的同源性很小。三个克隆含有与c-fes/fps原癌基因、重组激活基因1或人血管生成因子基因同源的区域。X射线诱导基因可能在受损细胞中发挥作用，调节DNA修复、细胞凋亡、突变和致癌作用。
Twelve x-ray-induced transcripts (xips), differentially expressed 8- to 230-fold in x-irradiated versus unirradiated radioresistant human melanoma (U1-Mel) cells, were isolated as cDNA clones (xip1 through xip12) after four rounds of differential hybridization. Northern analyses revealed rare, medium, and abundant xips, ranging in size from 1.2 to 10 kb. All transcripts were transiently expressed and induced by low, but not by high (>600 cGy), doses of radiation. Three transcripts (xip4, -7, and -12) were induced only by ionizing radiation, and many (i.e., xip1, -2, -3, -5, -6, -8, -9, -10, and -11) were also induced by UV irradiation or phorbol 12-myristate 13-acetate. Heat shock did not induce any of the xips, but it decreased basal levels of xip4, -7, -11, and -12. Three xip cDNA clones were identified as encoding thymidine kinase, DT diaphorase, and tissue-type plasminogen activator. The remaining nine cDNA clones showed little homology to known genes. Three clones contained regions homologous to c-fes/fps protooncogene, recombination activating gene 1, or the human angiogenesis factor gene. X-ray-inducible genes may function in damaged cells to regulate DNA repair, apoptosis, mutagenesis, and carcinogenesis.