A common major surface antigen on amastigotes and promastigotes of Leishmania species.
A common major surface antigen on amastigotes and promastigotes of Leishmania species.
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DOI：
10.1084/jem.162.3.902
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发表时间：
1985-09-01
影响因子：
15.3
通讯作者：
Nogueira, N
中科院分区：
文献类型：
--
作者：
Colomer-Gould, V;Glvao Quintao, L;Keithly, J;Nogueira, N
酶表面碘化和[35S]蛋氨酸生物合成标记，结合不同类型利什曼原虫患者血清的免疫沉淀，发现9种利什曼原虫和分离株的前鞭毛体和无鞭毛体的免疫优势部分为65,000 mR糖蛋白。一种形式的利什曼病患者的血清强烈识别这种成分，不仅在同源物种中，而且在异源物种中也是如此。除了免疫血清显示的交叉反应外，所有利什曼原虫共有的65,000 mR糖蛋白(GP)在非还原条件下在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中表现出向mR 50,000的特征转变。这些结果与我们之前的研究一致(7)，其中获得了几个杜诺瓦尼乳杆菌地理分离株的简单和相似的图谱，其主要表面糖蛋白65,000 mR显示出与这里描述的相同的特征。克利夫兰图谱显示6种利什曼原虫主要65,000个MR GP的结构相似性。提示免疫特异性可能是由于该分子糖基化的微小差异所致。根据最近的数据(13-15)，不同利什曼原虫之间的交叉保护已经通过辐射的整个前鞭毛体预防性免疫而获得，这种糖蛋白可能是用于所有形式的利什曼原虫免疫预防的抗原的一个很好的候选。
Enzymatic surface iodination and biosynthetic labeling with [35S]methionine, combined with immunoprecipitation by sera from patients with different forms of Leishmaniasis revealed a 65,000 Mr glycoprotein as the immunodominant moiety in promastigotes and amastigotes of the nine Leishmania species and isolates examined. Sera from patients with one form of Leishmaniasis recognized this component strongly, not only in the homologous, but also in the heterologous species. In addition to the crossreactivity displayed by immune sera, the 65,000 Mr glycoprotein (gp) common to all Leishmania species presented a characteristic shift to Mr 50,000 when samples were run in sodium dodecyl sulfate-polyacrylamide gel electrophoresis under nonreducing conditions. These results are in agreement with our previous studies (7), where a simple and similar profile was obtained for several geographic isolates of L. donovani, with a major surface glycoprotein of 65,000 Mr displaying the same characteristics described here. The structural similarity of the major 65,000 Mr gp of the six Leishmania species was demonstrated by Cleveland mapping. It is suggested that immunological specificities may be contributed by minor differences in glycosylation of this molecule. In keeping with recent data (13-15), where strong cross protection among different Leishmania species has been obtained by prophylactic immunization with irradiated whole promastigotes, this glycoprotein may be a good candidate for an antigen to be used for immunoprophylaxis of all forms of Leishmaniasis.