VIPP1, a nuclear gene of Arabidopsis thaliana essential for thylakoid membrane formation
VIPP1, a nuclear gene of Arabidopsis thaliana essential for thylakoid membrane formation
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DOI：
10.1073/pnas.061500998
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发表时间：
2001-03-27
影响因子：
11.1
通讯作者：
Westhoff, P
中科院分区：
文献类型：
--
作者：
Kroll, D;Meierhoff, K;Westhoff, P
光合作用过程中光能转化为化学能的过程定位于植物叶绿体的类囊体膜网络中。虽然已经描述了几种靶蛋白进入和穿过类囊体的途径，但对该膜系统的起源或类囊体的脂质骨架如何运输并与靶膜融合知之甚少。类囊体的生物发生和维持似乎涉及膜元件通过囊泡运输的流动。在这里，我们通过突变分析表明，一个单一的基因称为VIPP 1（质体中的囊泡诱导蛋白1）的缺失是有害的类囊体膜的形成。虽然VIPP 1是一种亲水性蛋白质，但它存在于内被膜和类囊体膜中。在VIPP 1缺失突变体中，囊泡形成被消除。我们建议，VIPP 1是必不可少的维持类囊体的运输途径，以前没有认识到。
The conversion of light to chemical energy by the process of photosynthesis is localized to the thylakoid membrane network in plant chloroplasts, Although several pathways have been described that target proteins into and across the thylakoids, little is known about the origin of this membrane system or how the lipid backbone of the thylakoids is transported and fused with the target membrane. Thylakoid biogenesis and maintenance seem to involve the flow of membrane elements via vesicular transport. Here we show by mutational analysis that deletion of a single gene called VIPP1 (vesicle-inducing protein in plastids 1) is deleterious to thylakoid membrane formation. Although VIPP1 is a hydrophilic protein it is found in both the inner envelope and the thylakoid membranes. In VIPP1 deletion mutants vesicle formation is abolished. We propose that VIPP1 is essential for the maintenance of thylakoids by a transport pathway not previously recognized.