Cleaning and Disinfection of Bacillus cereus Biofilm.
Cleaning and Disinfection of Bacillus cereus Biofilm.
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DOI：
10.5731/pdajpst.2014.005165
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发表时间：
2016-05-01
影响因子：
--
通讯作者：
Schwarz, John Spencer
中科院分区：
文献类型：
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作者：
Deal, Amanda;Klein, Dan;Schwarz, John Spencer
随着生物被膜修复的困难继续得到高度重视，消毒剂对细菌单物种生物被膜的测试方法一直在发展。细菌单物种生物膜污染对良好的生产实践规范的行业构成了真正的风险。然而，混合物种生物膜和含有细菌孢子的生物膜对清洁和消毒仍然是一个更大的挑战。在制药领域经常遇到的芽胞形成微生物中，蜡状芽孢杆菌的芽胞往往被确定为最难消毒和根除的。对消毒敏感度低的原因之一是这些孢子能够被包裹在胞外多糖生物膜基质中。在这一系列实验中，我们评估了蜡状芽孢杆菌生物膜相对于分离的蜡状芽孢杆菌孢子和来自非芽胞形成物种的生物膜的消毒敏感性。此外，我们评估了预清洁对增加这种敏感性的影响。LAY摘要：随着生物被膜修复的困难继续得到关注，消毒剂对细菌单物种生物被膜的测试方法一直在发展。细菌单物种生物膜污染对良好的生产实践规范的行业构成了真正的风险。然而，混合物种生物膜和含有细菌孢子的生物膜对清洁和消毒仍然是一个更大的挑战。在制药领域经常遇到的芽胞形成微生物中，蜡状芽孢杆菌的芽胞往往被确定为最难消毒和根除的。对消毒敏感度低的原因之一是这些孢子能够被包裹在胞外多糖生物膜基质中。在这一系列实验中，我们评估了蜡状芽孢杆菌生物膜相对于分离的蜡状芽孢杆菌孢子和来自非芽胞形成物种的生物膜的消毒敏感性。此外，我们评估了预清洁对增加这种敏感性的影响。
Methodology has been evolving for the testing of disinfectants against bacterial single-species biofilms, as the difficulty of biofilm remediation continues to gain much-needed attention. Bacterial single-species biofilm contamination presents a real risk to good manufacturing practice-regulated industries. However, mixed-species biofilms and biofilms containing bacterial spores remain an even greater challenge for cleaning and disinfection. Among spore-forming microorganisms frequently encountered in pharmaceutical manufacturing areas, the spores of Bacillus cereus are often determined to be the hardest to disinfect and eradicate. One of the reasons for the low degree of susceptibility to disinfection is the ability of these spores to be encapsulated within an exopolysachharide biofilm matrix. In this series of experiments, we evaluated the disinfectant susceptibility of B. cereus biofilms relative to disassociated B. cereus spores and biofilm from a non-spore-forming species. Further, we assessed the impact that pre-cleaning has on increasing that susceptibility.LAY ABSTRACT: Methodology has been evolving for the testing of disinfectants against bacterial single-species biofilms, as the difficulty of biofilm remediation continues to gain much-needed attention. Bacterial single-species biofilm contamination presents a real risk to good manufacturing practice-regulated industries. However, mixed-species biofilms and biofilms containing bacterial spores remain an even greater challenge for cleaning and disinfection. Among spore-forming microorganisms frequently encountered in pharmaceutical manufacturing areas, the spores of Bacillus cereus are often determined to be the hardest to disinfect and eradicate. One of the reasons for the low degree of susceptibility to disinfection is the ability of these spores to be encapsulated within an exopolysachharide biofilm matrix. In this series of experiments, we evaluated the disinfectant susceptibility of B. cereus biofilms relative to disassociated B. cereus spores and biofilm from a non-spore-forming species. Further, we assessed the impact that pre-cleaning has on increasing that susceptibility.