EFFECT OF BLOOD PLATELETS ON PROTHROMBIN UTILIZATION OF DOG AND HUMAN PLASMAS
EFFECT OF BLOOD PLATELETS ON PROTHROMBIN UTILIZATION OF DOG AND HUMAN PLASMAS
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DOI：
10.1152/ajplegacy.1949.159.2.316
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发表时间：
1949-01-01
影响因子：
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通讯作者：
BRINKHOUS, KM
中科院分区：
文献类型：
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作者：
BUCKWALTER, JA;BLYTHE, WB;BRINKHOUS, KM
测定了含有不同数量的血小板的狗和人血浆的凝血率。在没有抗凝剂的情况下，在2摄氏度的硅胶处理设备中，通过血液离心法将血小板数量降低到所需水平。测定凝血时间和凝血酶原利用率。在犬血浆中，当血小板含量低于原值的约35%时，凝血酶原利用率，即通过两阶段凝血酶原试验从血液或血浆样本中测定的凝血酶原消失率，会降低。人体血浆的可比值为25%。凝血时间很短。除非血小板降至原始数量的5-15%以下，否则不能提示凝血功能受损。在所有的费用中。人类样本比狗样本需要更少的血小板才能达到正常的凝血率。结论是，在这两种血浆中，决定凝血率的因素都不是血小板的数量。然而，如果血小板的数量减少到特定的临界水平以下，那么它们确实会成为凝血率的限制因素。
The rate of clotting of dog and human plasmas containing varying numbers of blood platelets was determined. Platelet numbers were reduced to desired levels by centrifugation of blood, without anticoagulant, in silicone-treated apparatus at 2[degree]C. Detns. of the clotting time and the prothrombin utilization rate were made on all blood and plasma samples. The prothrombin utilization rate, i.e., the rate of disappearance of prothrombin from the blood or plasma sample as determined by the 2-stage prothrombin test, was reduced in dog plasmas when the platelet content was below about 35% of the original values. The comparable value for human plasmas was 25%. Clotting time detns. failed to indicate impairment in clotting unless platelets were reduced to below 5-15% of their original numbers. In all expts. human samples required fewer platelets than dog samples for a normal clotting rate. It is concluded that in neither plasma is the number of platelets the factor which determines the rate of clotting. However, platelets do become a limiting factor in the clotting rate if their number is reduced below certain critical levels.