Synthesis and properties of a novel artificial nucleoside that acts as a redox switch.
Synthesis and properties of a novel artificial nucleoside that acts as a redox switch.
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作为氧化还原开关的新型人工核苷的合成和特性。
DOI：
10.1093/nass/nrp054
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
S. Obika
中科院分区：
文献类型：
--
作者：
Takeshi Baba;T. Kodama;T. Imanishi;S. Obika
寡核苷酸可以对外界因素可逆地改变双链形成能力，具有潜在的应用于各种基因组技术的潜力，如反义和基因诊断技术。设计并合成了一种新型的桥联核苷，该核苷能够感知氧化剂和还原剂。核苷在C2‘和C4’位之间形成二硫键，这将增强其在氧化条件下形成双链的能力。相反，二硫键在还原条件下被切割，可能导致双链形成能力的下降。
Oligonucleotide that can reversibly alter the duplex-forming ability in response to an external factor has potential to be applied to various genome technologies, e.g. antisense and gene diagnosis technology. A novel bridged nucleoside which is capable of sensing an oxidant and reductant was designed and synthesized. The nucleoside formed a disulfide bridge between C2' and C4' positions, which would enhance its duplex-forming ability under oxidative conditions. Contrastingly, the disulfide bridge was cleaved under reductive conditions; it could cause decrease of duplex-forming ability.