Group A streptococcus (GAS) carbohydrate as an immunogen for protection against GAS infection
Group A streptococcus (GAS) carbohydrate as an immunogen for protection against GAS infection
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DOI：
10.1086/498618
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发表时间：
2006-01-01
影响因子：
6.4
通讯作者：
Zabriskie, JB
中科院分区：
文献类型：
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作者：
Sabharwal, H;Michon, F;Zabriskie, JB
先前的研究表明，含有抗 A 族链球菌碳水化合物 (GAS CHO) 抗体的人血清对不同的 M 蛋白携带血清型具有调理作用。为了研究抗 GAS CHO 抗体在被动和主动保护中的作用，用与破伤风类毒素缀合的 GAS CHO 皮下或鼻内免疫小鼠，并在用活 GAS 攻击后监测死亡率和口腔定植情况。与对照小鼠相比，免疫小鼠显着免受 GAS 的全身或鼻部攻击。此外，对墨西哥儿童血清样本和咽喉培养物的研究揭示了抗 GAS CHO 抗体的高血清滴度与咽喉中 GAS 的存在之间存在负相关关系。还测试了抗 GAS CHO 抗体与人体组织和细胞骨架蛋白的交叉反应性。在任一测定中均未观察到交叉反应。目前的研究表明，GAS CHO 具有免疫原性并能预防 GAS 感染。
Previous studies have shown that human serum containing anti-group A streptococcus carbohydrate ( GAS CHO) antibodies were opsonic for different M protein-carrying serotypes. To investigate the role that anti GAS CHO antibodies play in passive and active protection, mice were immunized subcutaneously or intranasally with GAS CHO conjugated to tetanus toxoid, and mortality and oral colonization were monitored after challenge with live GAS. Compared with control mice, immunized mice were significantly protected against systemic or nasal challenge with GAS. Furthermore, studies of serum samples and throat cultures from Mexican children revealed an inverse relationship between high serum titers of anti-GAS CHO antibodies and the presence of GAS in the throat. Anti-GAS CHO antibodies were also tested for cross-reactivity with human tissues and cytoskeletal proteins. No cross-reactivity was observed in either assay. The present study demonstrates that GAS CHO is both immunogenic and protective against GAS infections.