Genomic DNA sequences of two new genes for new storage protein glutelin in rice.

Genomic DNA sequences of two new genes for new storage protein glutelin in rice.
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水稻新储存蛋白谷蛋白的两个新基因的基因组 DNA 序列。

DOI:
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发表时间:
1991
期刊:
Idengaku zasshi
影响因子:
--
通讯作者:
K. Oono
K. Oono
中科院分区:
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文献类型:
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作者:
F. Takaiwa;K. Oono

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分离了一个新的水稻储存蛋白谷氨酸cDNA和两个编码谷氨酸的基因组基因,并对其进行了测序。其中一个基因(GluA-3)与新cDNA的核苷酸序列完全一致,另一个基因(GluA-4)为假基因。这些谷蛋白基因彼此密切相关,属于A亚家族,包含I型(GluA-1)和II型(GluA-2)谷蛋白基因。利用合成的GluA-3基因特异性寡核苷酸作为探针,Northern blot分析表明,该基因在种子成熟过程中比其他谷蛋白基因表达得更早。
A new cDNA and two genomic genes encoding the rice storage protein glutelin were isolated and sequenced. The nucleotide sequence of one gene (GluA-3) was completely identical with that of the new cDNA identified here, and the other (GluA-4) was a pseudogene. These glutelin genes were closely related to each other, and belonged to the subfamily A containing the type I (GluA-1) and II (GluA-2) glutelin genes. The Northern blot analysis, using synthetic oligonucleotide specific to the GluA-3 gene as a probe, showed that this gene was expressed earlier than other glutelin genes during seed maturation.
DOI: 10.1016/0378-1119(84)90153-7
发表时间: 1984-01-01
期刊: GENE
影响因子: 3.5
作者:
HENIKOFF, S
通讯作者: HENIKOFF, S