ELECTRO-PHYSIOLOGICAL ANALYSIS OF NEURONAL THERMOSENSITIVITY IN RAT PREOPTIC AND HYPOTHALAMIC TISSUE-CULTURES
ELECTRO-PHYSIOLOGICAL ANALYSIS OF NEURONAL THERMOSENSITIVITY IN RAT PREOPTIC AND HYPOTHALAMIC TISSUE-CULTURES
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DOI：
10.1113/jphysiol.1982.sp014226
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发表时间：
1982-01-01
影响因子：
5.5
通讯作者：
GELLER, HM
中科院分区：
文献类型：
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作者：
BALDINO, F;GELLER, HM
对大鼠下丘脑基底内侧区（MBH）和视前区（POAH）的46个神经元（96个）进行热刺激。神经元对温度的反应是根据从平均自发活动与浴温相关的回归线计算的外推Q10确定的。热刺激（28 - 41 ℃）C）导致鉴定出3个不同的神经元组：温度不敏感的（0.5 ≤ C）神经元组; Q10 ≤温敏型（Q10 > 2）和冷敏型（Q10 < 0.5）。在MBH培养物中未发现温度敏感神经元。在有效地阻断突触传递的介质（12 mM-Mg 2+和0.25 mM-Ca 2+）的存在下，保持了温敏和冷敏神经元的敏感性。温度敏感性是体外视前神经元网络的一个特征，也是单个神经元的内在特征。
Neurons (96) in 46 explant tissue cultures of rat medial basal hypothalamus (MBH) and preoptic area (POAH) were subjected to thermal stimulation. Neuronal response to temperature was determined on the basis of an extrapolated Q10 calculated from the regression line relating mean spontaneous activity to bath temperature. Thermal stimulation (28-41.degree. C) of POAH cultures resulted in the identification of 3 distinct groups of neurons: temperature-insensitive (0.5 .ltoreq. Q10 .ltoreq. 2), warm-sensitive (Q10 > 2) and cold-sensitive (Q10 < 0.5). No temperature-sensitive neurons were identified in MBH cultures. In the presence of a medium which effectively blocks synaptic transmission (12 mM-Mg2+ and 0.25 mM-Ca2+) the sensitivity of both warm- and cold-sensitive neurons was preserved. Thermosensitivity is a characteristic of the preoptic neuronal network in vitro and is an intrinsic characteristic of individual neurons.