Theta-Burst Transcranial Magnetic Stimulation Alters Cortical Inhibition
Theta-Burst Transcranial Magnetic Stimulation Alters Cortical Inhibition
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DOI：
10.1523/jneurosci.1379-10.2011
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发表时间：
2011-01-26
影响因子：
5.3
通讯作者：
Funke, Klaus
中科院分区：
文献类型：
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作者：
Benali, Alia;Trippe, Joern;Funke, Klaus
人类皮层兴奋性可以通过重复经颅磁刺激（rTMS）来改变，但细胞机制在很大程度上是未知的。在这里，我们表明，模式的θ-突发刺激（TBS）（连续与间歇性）的交付不同的修改大鼠新皮层的电活动和蛋白质表达。间歇性TBS（iTBS），而不是连续TBS（cTBS），增强自发神经元放电和EEG伽马波段功率。感觉诱发的皮层抑制增加后iTBS，虽然TBS协议增加了第一感觉反应所产生的休息皮层状态。钙结合蛋白小清蛋白（PV）和钙结合蛋白D-28 k（CB）的皮质表达变化表明，rTMS后自发和诱发的皮质活动变化部分与抑制系统活性的改变有关。通过减少PV表达的快速尖峰的中间神经元，iTBS主要影响抑制控制的锥体细胞输出活动，而cTBS，通过减少CB表达，更可能影响树突整合的突触输入控制的其他类别的抑制性中间神经元。由另一类中间神经元表达的第三种主要的钙结合蛋白Calretinin完全不受影响。我们的结论是，不同模式的TBS调制不同的抑制性细胞类的活动，可能取决于这些抑制性神经元的突触连接和首选的放电模式。
Human cortical excitability can be modified by repetitive transcranial magnetic stimulation (rTMS), but the cellular mechanisms are largely unknown. Here, we show that the pattern of delivery of theta-burst stimulation (TBS) (continuous versus intermittent) differently modifies electric activity and protein expression in the rat neocortex. Intermittent TBS (iTBS), but not continuous TBS (cTBS), enhanced spontaneous neuronal firing and EEG gamma band power. Sensory evoked cortical inhibition increased only after iTBS, although both TBS protocols increased the first sensory response arising from the resting cortical state. Changes in the cortical expression of the calcium-binding proteins parvalbumin (PV) and calbindin D-28k (CB) indicate that changes in spontaneous and evoked cortical activity following rTMS are in part related to altered activity of inhibitory systems. By reducing PV expression in the fast-spiking interneurons, iTBS primarily affected the inhibitory control of pyramidal cell output activity, while cTBS, by reducing CB expression, more likely affected the dendritic integration of synaptic inputs controlled by other classes of inhibitory interneurons. Calretinin, the third major calcium-binding protein expressed by another class of interneurons was not affected at all. We conclude that different patterns of TBS modulate the activity of inhibitory cell classes differently, probably depending on the synaptic connectivity and the preferred discharge pattern of these inhibitory neurons.