MUTATION OF THE HAMSTER-CELL CYCLE GENE RCC1 IS COMPLEMENTED BY THE HOMOLOGOUS GENES OF DROSOPHILA AND SACCHAROMYCES-CEREVISIAE
MUTATION OF THE HAMSTER-CELL CYCLE GENE RCC1 IS COMPLEMENTED BY THE HOMOLOGOUS GENES OF DROSOPHILA AND SACCHAROMYCES-CEREVISIAE
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DOI：
10.1002/j.1460-2075.1991.tb08068.x
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发表时间：
1991-05-01
期刊：
影响因子：
11.4
通讯作者：
NISHIMOTO, T
中科院分区：
文献类型：
--
作者：
OHTSUBO, M;YOSHIDA, T;NISHIMOTO, T
RCC1 基因已从多种脊椎动物中分离出来，包括人类、仓鼠和非洲爪蟾。 与 RCC1 相似的基因，即 BJ1 和 SRM1/PRP20，已从昆虫果蝇和芽殖酵母酿酒酵母中分离出来。 仓鼠 BHK21 细胞系 tsBN2 中 RCC1 基因的突变赋予多效性表型，包括 G1 停滞和在 G1/S 边界同步的细胞有丝分裂的过早诱导。 同样，SRM1/PRP20 基因的突变是多效性的：srm1 突变体显示 G1 停滞并抑制缺乏信息素受体的突变体的交配缺陷，而 prp20 突变体显示 mRNA 代谢的改变。 在这里，我们表明 BJ1 和 SRM1/PRP20 都补充了 tsBN2 细胞的温度敏感表型。 与脊椎动物的 RCC1 蛋白一样，果蝇和酵母 RCC1 同源物的蛋白产物也位于哺乳动物细胞的细胞核中。 这些结果表明BJ1和SRM1/PRP20基因在功能上与脊椎动物的RCC1基因等效，并且RCC1基因在真核细胞周期中基因表达的调控中发挥着重要作用。
The RCC1 gene has been isolated from several vertebrates, including human, hamster and Xenopus. Genes similar to RCC1, namely BJ1 and SRM1/PRP20, have been isolated from the insect Drosophila and from the budding yeast Saccharomyces cerevisiae. A mutation of the RCC1 gene in the hamster BHK21 cell line, tsBN2, confers pleiotropic phenotypes, including G1 arrest and premature induction of mitosis in cells synchronized at the G1/S boundary. Similarly, mutations of the SRM1/PRP20 gene are pleiotropic: the srm1 mutant shows G1 arrest and suppression of the mating defect of mutants lacking pheromone receptors, and the prp20 mutant shows an alteration in mRNA metabolism. Here we show that both BJ1 and SRM1/PRP20 complement the temperature sensitive phenotype of the tsBN2 cells. Like RCC1 proteins of vertebrates, the protein products of the Drosophila and yeast RCC1 homologues were located in the nuclei of the mammalian cells. These results suggest that the BJ1 and SRM1/PRP20 genes are functionally equivalent to the vertebrate RCC1 genes, and that the RCC1 gene plays an important role in the regulation of gene expression in the eukaryotic cell cycle.