Octacalcium phosphate crystals including a higher density dislocation improve its materials osteogenecity
Octacalcium phosphate crystals including a higher density dislocation improve its materials osteogenecity
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DOI：
10.1016/j.apmt.2021.101279
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发表时间：
2021-12-02
影响因子：
8.3
通讯作者：
Suzuki, Osamu
中科院分区：
文献类型：
--
作者：
Hamai, Ryo;Sakai, Susumu;Suzuki, Osamu
在这里，我们表明，增强的成骨能力的磷酸八钙（OCP）生物材料，最近被确定为一个重要的元素，在混合的有机-无机纳米复合材料参与在哺乳动物骨骼中的初始羟基磷灰石晶体膨胀，结果从增强的化学性质，源于晶格应变和位错的存在。两种类型的OCP合成的存在下（c-OCP）和不存在下（w-OCP）的明胶，分别通过湿化学处理，并进行结构，化学和生物学分析。高分辨率透射电子显微镜（HRTEM）和快速傅里叶变换（FFT）分析表明，c-OCP晶体包含约6倍高的边缘位错与伯格斯矢量垂直于a轴的情况下，比w-OCP。在tris-HCl缓冲液中，c-OCP晶体的溶解倾向于晶体的长轴方向，最有可能是沿着c轴方向的晶格应变方向，而w-OCP晶体的溶解倾向于a轴方向。研究表明，较高的位错密度增加了内能，通过降低活化能，促进了c-OCP的溶解和水解。与w-OCP晶体相比，c-OCP晶体在体外增强了间充质干细胞2D和3D球状体的分化、体内骨形成以及在临界尺寸大鼠颅骨缺损模型中的磷灰石晶体学取向，同时比w-OCP晶体更早地转化为磷灰石结构。目前的研究表明，与位错相关的溶解沿着OCP转化的增强是骨诱导的决定因素，这可能与使用OCP生物材料的正常骨发育有关。(c)2021作者由Elsevier Ltd.发布。这是一个CC BY-NC-ND许可证下的开放获取文章（http://creativecommons.org/licenses/by-nc-nd/4.0/）
Herein, we show that the enhanced osteogenecity of octacalcium phosphate (OCP) biomaterial, recently identified as an important element in hybrid organic-inorganic nanocomposites involved in the initial hydroxyapatite crystal expansion in mammal bones, results from an enhanced chemical property, stemming from the presence of lattice strain and dislocations. Two types of OCPs were synthesized by wet-chemical processing in the presence (c-OCP) and absence (w-OCP) of gelatin, respectively, and subjected to structural, chemical, and biological analyses. High-resolution transmission electron microscopy (HRTEM) and fast Fourier transform (FFT) analyses revealed that c-OCP crystals contained approximately six times higher edge dislocations with Burgers vectors perpendicular to a-axis than that in the case of w-OCP. The dissolution of c-OCP crystal in tris-HCl buffer occurred toward the long axis of the crystal, most likely, toward the lattice strain along the c-axis direction, while w-OCP crystal dissolved toward the a axis direction. The study suggested that the increment of internal energy by the higher dislocation density contributed promoting c-OCP dissolution and hydrolysis through decreasing the activation energy. c-OCP crystal displayed enhanced in vitro mesenchymal stem 2D cell and 3D spheroid differentiation, in vivo bone formation, and apatite crystallographic orientation in critical-sized rat calvarial defect model as compared to w-OCP crystal, at the same time, converting to apatite structure earlier than w-OCP. The present study demonstrates that dislocation-related dissolution along with enhanced conversion of OCP is a determinant in bone induction, which may be relevant to normal bone development utilizing OCP biomaterials. (c) 2021 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )