Role of Hexahistidine in Directed Nanoassemblies of Tobacco Mosaic Virus Coat Protein
Role of Hexahistidine in Directed Nanoassemblies of Tobacco Mosaic Virus Coat Protein
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DOI：
10.1021/nn1025719
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发表时间：
2011-03-01
期刊：
影响因子：
17.1
通讯作者：
Blum, Amy Szuchmacher
中科院分区：
文献类型：
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作者：
Bruckman, Michael A.;Soto, Carissa M.;Blum, Amy Szuchmacher
纳米技术的一个共同挑战是制造具有明确定义的纳米结构和性能的材料。在这里，我们报告的基因工程烟草花叶病毒（TMV）的外壳蛋白（CP），其中一个六组氨酸（His）标签被纳入，可以自组装成磁盘，六角排列的磁盘，堆叠磁盘，螺旋杆，纤维，和细长的筏。与野生型WT-TMV-CP相比，在TMV-CP的C-末端插入His标签显示出显著影响自组装。此外，归因于His-TMV-CP的替代自组装的His标签相互作用可以通过添加乙醇和镍-次氮基三乙酸（Ni-NTA）来控制，如用原子力显微镜监测的。
A common challenge in nanotechnology is the fabrication of materials with well-defined nanoscale structure and properties. Here we report that a genetically engineered tobacco mosaic virus (TMV) coat protein (CP), to which a hexahistidine (His) tag was incorporated, can self-assemble Into disks, hexagonally packed arrays of disks, stacked disks, helical rods, fibers, and elongated rafts. The insertion of a His tag to the C-terminus of TMV-CP was shown to significantly affect the self-assembly In comparison to the wild type, WT-TMV-CP. Furthermore, the His tag Interactions attributed to the alternative self assembly of His-TMV-CP can be controlled through ethanol and nickel-nitrilotriacetic acid, (Ni-NTA) additions as monitored with atomic force microscopy.