Generation of Mouse Bone Marrow-Derived Macrophages
Generation of Mouse Bone Marrow-Derived Macrophages
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DOI：
10.1007/978-1-61779-527-5_12
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发表时间：
2012-01-01
期刊：
影响因子：
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通讯作者：
Manzanero, Silvia
中科院分区：
文献类型：
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作者：
Manzanero, Silvia
从小鼠组织中分离常驻巨噬细胞涉及复杂的程序，产量很小。这对于许多功能性巨噬细胞测定是不方便的，其需要大量相对同质的细胞。另一种方法是在体外用适当的生长因子培养骨髓细胞，使前体细胞分化成大量的巨噬细胞。除了使用M-CSF（巨噬细胞集落刺激因子）之外，该方法简单且便宜，并且其特征在于高产率和可重复性。一旦获得，骨髓源性巨噬细胞（BBM）可用于相当数量的功能和结构测定，通常被视为固有巨噬细胞在先天免疫系统中的作用的模型。
Isolation of resident macrophages from mouse tissues involves complex procedures for a small yield. This is inconvenient for many functional macrophage assays, which require large numbers of relatively homogeneous cells. An alternative method is the culture of bone marrow cells in vitro with appropriate growth factors, to allow the differentiation of precursor cells into large numbers of macrophages. This procedure is easy and inexpensive except for the use of M-CSF, the macrophage colony stimulating factor, and it is characterised by high yield and reproducibility. Once obtained, bone marrow-derived macrophages (BMMs) can be used for a considerable number of functional and structural assays and are commonly regarded as a model for the role of resident macrophages in the innate immune system.