Structural Basis for the Bidirectional Activity of Bacillus nanoRNase NrnA.
Structural Basis for the Bidirectional Activity of Bacillus nanoRNase NrnA.
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DOI：
10.1038/s41598-017-09403-x
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发表时间：
2017-09-11
影响因子：
4.6
通讯作者：
Malhotra A
中科院分区：
文献类型：
--
作者：
Schmier BJ;Nelersa CM;Malhotra A
NanoRNAs are RNA fragments 2 to 5 nucleotides in length that are generated as byproducts of RNA degradation and abortive transcription initiation. Cells have specialized enzymes to degrade nanoRNAs, such as the DHH phosphoesterase family member NanoRNase A (NrnA). This enzyme was originally identified as a 3′ → 5′ exonuclease, but we show here that NrnA is bidirectional, degrading 2–5 nucleotide long RNA oligomers from the 3′ end, and longer RNA substrates from the 5′ end. The crystal structure of Bacillus subtilis NrnA reveals a dynamic bi-lobal architecture, with the catalytic N-terminal DHH domain linked to the substrate binding C-terminal DHHA1 domain via an extended linker. Whereas this arrangement is similar to the structure of RecJ, a 5′ → 3′ DHH family DNase and other DHH family nanoRNases, Bacillus NrnA has gained an extended substrate-binding patch that we posit is responsible for its 3′ → 5′ activity.
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DOI：
10.1126/science.1174343
发表时间：
2009-08-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Guo M;Chong YE;Beebe K;Shapiro R;Yang XL;Schimmel P
通讯作者：
Schimmel P
影响因子：
14.9
作者：
Fang M;Zeisberg WM;Condon C;Ogryzko V;Danchin A;Mechold U
通讯作者：
Mechold U
影响因子：
5.8
作者：
Gouet, P;Courcelle, E;Métoz, F
通讯作者：
Métoz, F
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
DOI：
10.1073/pnas.1010436107
发表时间：
2010-11-23
影响因子：
11.1
作者：
Hyde, Samantha J.;Eckenroth, Brian E.;Doublie, Sylvie
通讯作者：
Doublie, Sylvie