A novel transgenic technique that allows specific marking of the neural crest cell lineage in mice
A novel transgenic technique that allows specific marking of the neural crest cell lineage in mice
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DOI：
10.1006/dbio.1999.9323
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发表时间：
1999-08-01
影响因子：
2.7
通讯作者：
Yamamura, K
中科院分区：
文献类型：
--
作者：
Yamauchi, Y;Abe, K;Yamamura, K
神经嵴细胞是胚胎的多能干细胞，其产生各种细胞/组织类型，因此作为用于研究细胞特化和细胞分化机制的良好模型系统。为了分析神经嵴细胞谱系，设计了一种通过Cre-loxP系统操纵小鼠基因组的有效方法。我们产生的转基因小鼠携带的Cre基因驱动的蛋白0（PO）的启动子。为了检测Cre介导的DNA重组，我们将PO-Cre转基因小鼠与CAG-CAT-Z指示转基因小鼠杂交。CAG-CAT-Z Tg系携带鸡β-肌动蛋白启动子下游的lacZ基因和侧翼为两个loxP序列的“填充物”片段，使得lacZ仅在通过Cre重组酶的作用去除填充物时表达。在三个不同的PO-Cre系与CAG-CAT-Z Tg，胚胎携带两个转基因显示lacZ表达的组织来源于神经嵴细胞，如脊髓背根神经节，交感神经系统，肠神经系统，和腹侧颅面间充质的阶段晚于9.0 dpc。这些发现为哺乳动物神经嵴细胞分化提供了一些见解。我们相信，PO-Cre转基因小鼠将有助于许多有趣的实验，包括谱系分析，纯化，和哺乳动物神经嵴细胞的遗传操作。(C)1999年，学术出版社。
Neural crest cells are embryonic, multipotent stem cells that give rise to various cell/tissue types and thus serve as a good model system for the study of cell specification and mechanisms of cell differentiation. For analysis of neural crest cell lineage, an efficient method has been devised for manipulating the mouse genome through the Cre-loxP system. We generated transgenic mice harboring a Cre gene driven by a promoter of protein 0 (PO). To detect the Cre-mediated DNA recombination, we crossed PO-Cre transgenic mice with CAG-CAT-Z indicator transgenic mice. The CAG-CAT-Z Tg line carries a lacZ gene downstream of a chicken beta-actin promoter and a "stuffer" fragment flanked by two loxP sequences, so that lacZ is expressed only when the stuffer is removed by the action of Cre recombinase. In three different PO-Cre lines tressed with CAG-CAT-Z Tg, embryos carrying both transgenes showed lacZ expression in tissues derived from neural crest cells, such as spinal dorsal root ganglia, sympathetic nervous system, enteric nervous system, and ventral craniofacial mesenchyme at stages later than 9.0 dpc. These findings give some insights into neural crest cell differentiation in mammals. We believe that PO-Cre transgenic mice will facilitate many interesting experiments, including lineage analysis, purification, and genetic manipulation of the mammalian neural crest cells. (C) 1999, Academic Press.