Dynamically Tunable Cell Culture Platforms for Tissue Engineering and Mechanobiology.
Dynamically Tunable Cell Culture Platforms for Tissue Engineering and Mechanobiology.
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DOI：
10.1016/j.progpolymsci.2016.09.004
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发表时间：
2017-02
影响因子：
27.1
通讯作者：
Kim DH
中科院分区：
文献类型：
--
作者：
Uto K;Tsui JH;DeForest CA;Kim DH
人体组织是许多不同细胞类型的复杂集合体，它们嵌入复杂但明确定义的细胞外基质（ECM）结构中。ECM中的动态生化、物理化学和机械结构变化定义并调节组织特异性细胞行为。为了概括这种复杂的体外环境，动态聚合物基生物材料已成为探测和指导细胞功能主动变化的有力工具。聚合化学、结构调节和加工技术的快速发展，以及刺激响应性的结合，现在允许合成微环境捕获天然组织的大部分动态复杂性。这些平台不仅由化学和分子上与ECM相似的天然聚合物组成，而且还由完全合成的聚合物组成。在这里，我们回顾了最近在体外的努力，从材料设计的角度来模拟包括天然组织ECM的动态微环境。我们还讨论了这些动态聚合物基生物材料是如何被用于基础细胞机械生物学研究，以及对组织工程和再生医学的努力。
Human tissues are sophisticated ensembles of many distinct cell types embedded in the complex, but well-defined, structures of the extracellular matrix (ECM). Dynamic biochemical, physicochemical, and mechano-structural changes in the ECM define and regulate tissue-specific cell behaviors. To recapitulate this complex environment in vitro, dynamic polymer-based biomaterials have emerged as powerful tools to probe and direct active changes in cell function. The rapid evolution of polymerization chemistries, structural modulation, and processing technologies, as well as the incorporation of stimuli-responsiveness, now permit synthetic microenvironments to capture much of the dynamic complexity of native tissue. These platforms are comprised not only of natural polymers chemically and molecularly similar to ECM, but those fully synthetic in origin. Here, we review recent in vitro efforts to mimic the dynamic microenvironment comprising native tissue ECM from the viewpoint of material design. We also discuss how these dynamic polymer-based biomaterials are being used in fundamental cell mechanobiology studies, as well as towards efforts in tissue engineering and regenerative medicine.