COVID-19 Plasma Extracellular Vesicles Increase the Density of Lipid Rafts in Human Small Airway Epithelial Cells.
COVID-19 Plasma Extracellular Vesicles Increase the Density of Lipid Rafts in Human Small Airway Epithelial Cells.
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DOI：
10.3390/ijms24021654
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发表时间：
2023-01-14
影响因子：
5.6
通讯作者：
Alli, Abdel A.
中科院分区：
文献类型：
--
作者：
Darwish, Sara;Liu, Lauren P.;Robinson, Tanya O.;Tarugu, Spurthi;Owings, Anna H.;Glover, Sarah C.;Alli, Abdel A.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）病毒是COVID-19疾病的病原体。COVID-19病毒感染可以影响许多细胞类型，包括肺和气道的上皮细胞。细胞外囊泡（EV）几乎由所有细胞类型释放，并且其包装的货物允许细胞间通讯、细胞分化和信号转导。来自病毒感染细胞的货物可包括病毒衍生的代谢物、miRNA、核酸和蛋白质。我们假设COVID-19血浆EV在被正常人小气道上皮细胞（SAEC）摄取后可以诱导形成称为脂筏的信号平台。来自有或没有COVID-19的患者的循环EV通过纳米颗粒跟踪分析、使用特异性抗体的蛋白质印迹和透射电子显微镜进行表征。正常人小气道上皮细胞的原代培养物用来自两个患者组的EV进行挑战，并且通过荧光显微镜测量脂筏形成，并通过蔗糖密度梯度分析进行评估。总的来说，我们的数据表明，来自COVID-19感染患者的循环EV可以诱导正常人小气道上皮细胞中脂筏的形成。这些结果表明，需要进一步研究，旨在调查是否增加密度的脂筏在这些细胞中促进病毒进入和受体细胞中的特定信号通路的改变。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus is the causative agent of the COVID-19 disease. COVID-19 viral infection can affect many cell types, including epithelial cells of the lungs and airways. Extracellular vesicles (EVs) are released by virtually all cell types, and their packaged cargo allows for intercellular communication, cell differentiation, and signal transduction. Cargo from virus-infected cells may include virally derived metabolites, miRNAs, nucleic acids, and proteins. We hypothesized that COVID-19 plasma EVs can induce the formation of signaling platforms known as lipid rafts after uptake by normal human small airway epithelial cells (SAECs). Circulating EVs from patients with or without COVID-19 were characterized by nanoparticle tracking analysis, Western blotting using specific antibodies, and transmission electron microscopy. Primary cultures of normal human small airway epithelial cells were challenged with EVs from the two patient groups, and lipid raft formation was measured by fluorescence microscopy and assessed by sucrose density gradient analysis. Collectively, our data suggest that circulating EVs from COVID-19-infected patients can induce the formation of lipid rafts in normal human small airway epithelial cells. These results suggest the need for future studies aimed at investigating whether the increased density of lipid rafts in these cells promotes viral entry and alteration of specific signaling pathways in the recipient cells.
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2018-08
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
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DOI：
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发表时间：
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影响因子：
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