Whole-Cell Fatty Acid Composition to Characterize and Differentiate Isolates of Rhizoctonia Species Associated with Turfgrass Diseases in Japan
Whole-Cell Fatty Acid Composition to Characterize and Differentiate Isolates of Rhizoctonia Species Associated with Turfgrass Diseases in Japan
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全细胞脂肪酸组成用于表征和区分与日本草坪病害相关的丝核菌属物种的分离株
DOI：
10.1007/pl00013043
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发表时间：
2002
影响因子：
1.2
通讯作者：
M. Hyakumachi
中科院分区：
文献类型：
--
作者：
A. Priyatmojo;R. Yamauchi;K. Kageyama;M. Hyakumachi
对34个丝核菌属菌株进行了细胞脂肪酸分析，并对它们进行了鉴定，其中包括双核丝核菌AG-D（Ⅰ）、AG-D（Ⅱ）、R. solaniAG 2-2 IIIB、AG 2-2 LP、R.与日本草坪草病害有关的卷叶变种（circinatavar）、卷叶变种（circinata）和稻变种（var. oryzae）。肉豆蔻酸，十五烷酸，棕榈酸，棕榈油酸，硬脂酸，油酸，亚油酸和亚麻酸始终存在于所有菌株中的不同数量。Rhizoctonia AG-D（I）、AG-D（II）、R. solaniAG 2-2 IIIB和AG 2- 2LP，而对R.棕榈酸、油酸和亚油酸是发现的主要脂肪酸，占全细胞脂肪酸含量的88.30-98.37%。剩余的脂肪酸以较小的量存在。在一个单一的组内的分离株紧密聚类，而从不同的群体分离株细胞脂肪酸的平均连锁聚类分析的基础上明确区分。主成分分析，所有的脂肪酸检测的基础上，证实了独特的分离代表六组ofRhizoctoniaspecies从草坪草。这些结果表明，脂肪酸分析是有用的鉴定和鉴别与草坪草病害相关的丝核菌种的菌株。
Cellular fatty acids were analyzed to characterize and differentiate 34 isolates ofRhizoctoniaspecies representing binucleateRhizoctoniaAG-D (I), AG-D (II),R. solaniAG 2-2 IIIB, AG 2-2 LP,R. circinatavar.circinataand var.oryzaeassociated with turfgrass diseases in Japan. Myristic, pentadecanoic, palmitic, palmitoleic, stearic, oleic, linoleic and linolenic acids were consistently present in varying quantities in all isolates. Heptadecanoic and 9-heptadecenoic acids were present in isolates ofRhizoctoniaAG-D (I), AG-D (II),R. solaniAG 2-2 IIIB and AG 2-2 LP but not in isolates ofR. circinatavar.circinataand var.oryzae.Palmitic, oleic and linoleic acids were the major fatty acids found, constituting 88.30-98.37% of the whole-cell fatty acid content. The remaining fatty acids were present in smaller amounts. Isolates within a single group were closely clustered, whereas isolates from different groups were clearly distinguishable based on average linkage cluster analysis of cellular fatty acids. Principal component analysis, based on all fatty acids detected, confirmed the distinct separation of isolates representing the six groups ofRhizoctoniaspecies obtained from turfgrasses. These results suggested that fatty acid analysis is useful for the characterization and differentiation of isolates ofRhizoctoniaspecies associated with turfgrass diseases.