Field survey of sex-reversals in the medaka, Oryzias latipes:: genotypic sexing of wild populations
Field survey of sex-reversals in the medaka, Oryzias latipes:: genotypic sexing of wild populations
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DOI：
10.2108/zsj.21.613
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发表时间：
2004-06-01
影响因子：
0.9
通讯作者：
Sakaizumi, M
中科院分区：
文献类型：
--
作者：
Shinomiya, A;Otake, H;Sakaizumi, M
青鳉具有XX/XY性别决定机制。DMY是一个Y连锁的DM结构域基因，它是通过定位克隆获得的性别决定基因的主要候选基因。此外，DMY在雄性发育过程中的关键作用是通过研究两个野生型XY雌性突变体建立的。本研究通过对野生鱼类基因型性别（DMY阴性或DMY阳性）的广泛调查，寻找新的DMY和性别决定相关基因突变。我们研究了来自日本40个地区的2274条野生捕捞鱼类，以及来自日本、韩国、中国和台湾的69个野生种群的730条鱼类。在13个和8个地点分别发现了26条DMY阳性（XY）雌性和15条DMY阴性（XX）雄性。将来自11个地区的16个XY性反转株与自交系的XY雄性交配，分析了F-1代的基因型和表型性别。所有这些XY性别逆转在F-1代产生XY雌性，并且所有F-1 XY雌性具有母体Y染色体。这些结果表明，DMY基因是野生群体中常见的性别决定基因。所有XY性反转的研究都有DMY或DMY连锁基因突变。
The medaka, Oryzias latipes, has an XX/XY sex determination mechanism. A Y-linked DM domain gene, DMY has been isolated by positional cloning as a prime candidate for the sex-determining gene. Furthermore, the crucial role of DMY during male development was established by studying two wild-derived XY female mutants. In this study, to find new DMY and sex-determination related gene mutations, we conducted a broad survey of the genotypic sex (DMY-negative or DMY-positive) of wild fish. We examined 2274 wild-caught fish from 40 localities throughout Japan, and 730 fish from 69 wild stocks from Japan, Korea, China, and Taiwan. The phenotypic sex type agreed with the genotypic sex of most fish, while 26 DMY-positive (XY) females and 15 DMY-negative (XX) males were found from 13 and 8 localities, respectively. Sixteen XY sex-reversals from 11 localities were mated with XY males of inbred strains, and the genotypic and phenotypic sexes of the F-1 progeny were analyzed. All these XY sex-reversals produced XY females in the F-1 generation, and all F-1 XY females had the maternal Y chromosome. These results show that DMY is a common sex-determining gene in wild populations of O. latipes and that all XY sex-reversals investigated had a DMY or DMY-linked gene mutation.