Reliability of rectal epithelial kinetic patterns as an intermediate biomarker of colon cancer.
Reliability of rectal epithelial kinetic patterns as an intermediate biomarker of colon cancer.
复制标题
直肠上皮动力学模式作为结肠癌中间生物标志物的可靠性。
DOI：
--
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
G. Gasbarrini
中科院分区：
文献类型：
--
作者：
M. Anti;G. Marra;A. Percesepe;F. Armelao;G. Gasbarrini
使用生物标志物评估癌症风险是基于癌症多步骤过程的模型；这些标记被认为反映了这一过程的早期阶段。因此，有效的风险生物标志物必须在正常和高风险受试者中表现出差异表达，以及与癌发生阶段的定量相关性。它还应该很容易在小组织样本中检测到，并且对化学预防剂的调节有反应。细胞增殖是研究最广泛的癌症风险标志物之一。病例对照研究表明，通过体外放射自显影或免疫组织化学技术在直肠粘膜活检中评估上皮细胞增殖参数，可以区分结肠癌正常风险和高风险人群。然而，我们最近回顾了 152 名受试者（43 名对照者、84 名腺瘤患者、25 名结肠癌切除者）的直肠活检样本，这些样本经过体外 3H-胸苷放射自显影处理，并尝试通过多元回归分析将各种增殖参数与临床和病理变量相关联。总隐窝标记指数 (LI) 的升高，特别是上隐窝 LI，与腺瘤性息肉的存在显着相关，尽管随后的线性判别分析显示，LI 区分息肉患者和对照的准确性实际上相当低。然而，我们还发现上隐窝 LI 是腺瘤性息肉复发的可靠预测因子。对 40 名结肠腺瘤患者进行息肉切除术后 2 年随访，对直肠增殖指数进行重复评估，结果显示出显着的稳定性。（摘要截短为 250 字）
The use of biomarkers to assess cancer risk is based on the model of cancer as a multistep process; such markers are assumed to reflect an early stage in this process. A valid biomarker of risk must therefore show differential expression in normal and high-risk subjects, as well as quantitative correlation with the stage of carcinogenesis. It should also be easy to detect in small tissue specimens and responsive to modulation by chemopreventive agents. Cell proliferation is one of the most widely investigated markers of cancer risk. Case-control studies have shown that epithelial cell proliferation parameters, assessed in rectal mucosal biopsies by means of in vitro autoradiographic or immunohistochemical techniques, can discriminate between populations with normal and high risks for colon cancer. However, we recently reviewed rectal biopsies from 152 subjects (43 controls, 84 with adenomas, 25 resected for colon cancer) processed for in vitro 3H-thymidine autoradiography, and attempted to correlate various proliferative parameters with clinical and pathological variables by means of multiple regression analysis. Elevations of total crypt labeling indices (LIs), particularly upper crypt LIs, were significantly associated with the presence of adenomatous polyps, although subsequent linear discriminant analysis revealed that the accuracy of LIs in discriminating between polyp patients and controls was actually quite low. However, we have also found that upper crypt LIs are reliable predictors of adenomatous polyp recurrence. Repeated evaluations of rectal proliferative indices over a 2-year post-polypectomy follow-up of 40 patients with colonic adenomas revealed substantial stability.(ABSTRACT TRUNCATED AT 250 WORDS)