Internalisation of cell-penetrating peptides into tobacco protoplasts
Internalisation of cell-penetrating peptides into tobacco protoplasts
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DOI：
10.1016/j.bbamem.2005.01.006
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发表时间：
2005-05-20
影响因子：
3.4
通讯作者：
Langel, Ü
中科院分区：
文献类型：
--
作者：
Mäe, M;Myrberg, H;Langel, Ü
细胞膜保护细胞不受周围环境的影响，这是大多数亲水分子无法穿透的。在过去的十年中，细胞穿透肽（CPPs）被发现和开发。CPPs进入哺乳动物细胞，携带分子量数倍于自身的货物分子穿过质膜。已知的植物细胞转化方法效率较低，有待改进。利用CPPs作为植物细胞内化的潜在递送载体的可能性已经在目前的工作中进行了研究。我们分析和比较了荧光素标记的CPPs、转运蛋白、TP10、穿透素和pVEC在Bowes人黑色素瘤细胞和烟草品种（cv.）中的摄取情况。SR-1原生质体（没有细胞壁的植物细胞）。我们用荧光显微镜、荧光光谱法和荧光活化细胞分选仪（FACS）研究了CPPs的内化效率。所有的方法首次表明，这些cps可以内化到烟草N. acacum cv中。SR-1原生质体。转运蛋白在哺乳动物细胞和植物原生质体中的吸收效率最高，植物原生质体对CPPS的内化可能为植物转染开辟了一条新的有效途径。(C) 2005 Elsevier B.V.版权所有
Cells are protected from the surrounding environment by plasma membrane which is impenetrable for most hydrophilic molecules. In the last 10 years cell-penetrating peptides (CPPs) have been discovered and developed. CPPs enter mammalian cells and carry cargo molecules over the plasma membrane with a molecular weight several times their own. Known transformation methods for plant cells have relatively low efficiency and require improvement. The possibility to use CPPs as potential delivery vectors for internalisation in plant cells has been studied in the present work. We analyse and compare the uptake of the fluorescein-labeled CPPs, transportan, TP10, penetratin and pVEC in Bowes human melanoma cells and Nicotiana tabacum cultivar (cv.) SR-1 protoplasts (plant cells without cell wall). We study the internalisation efficiency of CPPs with fluorescence microscopy, spectrofluorometry and fluorescence-activated cell sorter (FACS). All methods indicate, for the first time, that these CPPs can internalise into N. tabacum cv. SR-1 protoplasts. Transportan has the highest uptake efficacy among the studied peptides, both in mammalian cells and plant protoplast, The internalisation of CPPS by plant protoplasts may open up a new effective method for transfection in plants. (C) 2005 Elsevier B.V. All rights reserved.