Free and bound volatiles of rocket (Eruca sativa Mill.)
Free and bound volatiles of rocket (Eruca sativa Mill.)
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DOI：
10.1002/ffj.1883
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发表时间：
2008-07-01
影响因子：
2.6
通讯作者：
Mastelic, Josip
中科院分区：
文献类型：
--
作者：
Blazevic, Ivica;Mastelic, Josip
火箭的挥发性成分(Eruca sativa Mill.)在Clevenger装置中，通过水蒸气蒸馏从自溶前后的新鲜植物材料和从干燥的植物材料中分离出带有绿豆的花和叶。用气相色谱/气相色谱-质谱仪(GC/GC-MS)对所有分离的挥发性化合物进行分析。鉴定出硫和/或氮化合物、脂肪酸和酯、脂肪醇和羰基化合物以及比例较小的其他一些化合物。主要的硫和/或氮组分是葡萄糖芥素降解挥发物：4-甲硫基丁基异硫氰酸酯(9.0-81.7%)和5-甲硫杂环戊腈(2.4-17.7%)。此外，还鉴定了脂肪酸和酯的主要成分，如：癸酸(1.2-32.8%)、十六酸(2.7-22.3%)、十二酸(0.6-8.7%)、(EE)-9，12-十八碳二烯酸(6.5%)、十四酸(1.5-6.4%)、亚麻酸甲酯(0.4-5.1%)和十六酸甲酯(0.3-1.2%)。从火箭花和绿豆叶的新鲜植物材料中分离得到挥发性化合物的O-糖苷。通过对糖苷的酶解及GC和GC-MS分析，首次鉴定出27个挥发性苷元。主要苷元有：2-苯乙醇(23.4-62.8%)、香草酸甲酯(5.4-12.4%)、苯甲醇(5.4-8.6%)、丁香酚(1.2-7.5%)、对苯甲醇(0.44.6%)、3-氧代-α-紫罗兰醇(0.4-3.2%)、4-氧代-β-紫罗兰醇(3.2%)、邻甲氧基苯酚(1.1-2.8%)。苯酚(0.3-2.8%)和反式香芹醇(0.2-2.3%)。通过比较，水蒸气蒸馏得到的挥发物和挥发的O-苷元馏分中只有(Z)-3-己烯-L-醇、2-甲基丁酸和2-苯乙醛。版权所有(C)2008 John Wiley&Sons，Ltd.
Volatile fractions of rocket (Eruca sativa Mill.) flowers and leaves with green beans were isolated by hydrodistillation in a Clevenger apparatus from fresh plant material prior to and after autolysis and from dried plant material. All isolates of volatile compounds were analysed by gas chromatography/gas chromatography-mass spectrometry (GC/GC-MS). Sulphur and/or nitrogen compounds, fatty acids and esters, aliphatic alcohols and carbonyl compounds and some other compounds in smaller percentages were identified. The main sulphur and/or nitrogen components were glucoerucin degradation volatiles: 4-methylthiobutyl isothiocyanate (9.0-81.7%) and 5-methylthiopentanenitrile (2.4-17.7%). Also, fatty acids and esters were identified as major components, such as: decanoic acid (1.2-32.8%), hexadecanoic acid (2.7-22.3%), dodecanoic acid (0.6-8.7%), (EE)-9,12-octadecadienoic acid (6.5%), tetradecanoic acid (1.5-6.4%), methyl linolenate (0.4-5.1%) and methyl hexadecanoate (0.3-1.2%). The O-glycosides of volatile compounds were isolated from fresh plant material of rocket flowers and leaves with green beans. After the enzymatic hydrolysis of glycosides and analysis by GC and GC-MS, 27 volatile aglycones were identified for the first time. The main aglycones were: 2-phenylethyl alcohol (23.4-62.8%), methyl vanillate (5.4-12.4%), benzyl alcohol (5.4-8.6%), eugenol (1.2-7.5%),p-anisyl alcohol (0.44.6 %), 3-oxo-alpha-ionol (0.4 -3.2 %), 4-oxo-beta-ionol (3.2 %), o-methoxy phenol (1.1-2.8 %), phenol (0.3-2.8 %) and trans-carveol (0.2-2.3%). When compared, only (Z)-3-hexen-l-ol, 2-methylbutanoic acid and 2-phenylacetaldehyde were present in volatiles obtained by hydrodistillation and the volatile O-aglycone fractions. Copyright (C) 2008 John Wiley & Sons, Ltd.