K+ preference at the NaK channel entrance revealed by fluorescence lifetime and anisotropy analysis of site-specifically incorporated (7-hydroxycoumarin-4-yl)ethylglycine
K+ preference at the NaK channel entrance revealed by fluorescence lifetime and anisotropy analysis of site-specifically incorporated (7-hydroxycoumarin-4-yl)ethylglycine
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通过荧光寿命和位点特异性掺入的 (7-羟基香豆素-4-基)乙基甘氨酸的各向异性分析揭示了 NaK 通道入口处的 K 偏好
DOI：
10.1039/c5cc06124e
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发表时间：
2015
影响因子：
4.9
通讯作者：
Tian Changlin
中科院分区：
文献类型：
--
作者：
Liu Sanling;Lv Pei;Li Dong;Guo Xiaoqi;Zhang Bo;Yu Mu;Li D;an;Xiong Ying;Zhang Longhua;Tian Changlin
将荧光非天然氨基酸（7-羟基香豆素-4-基）乙基甘氨酸（HC）位点特异性掺入Phe 69位点，靠近NaK通道选择性过滤器的入口。在具有高K+/Na+摩尔比的缓冲液中NaK-F69 HC的荧光寿命降低和时间分辨各向异性升高表明在NaK通道的入口处的K+偏好，与NaK通道的先前晶体结构结果一致。
The fluorescent unnatural amino acid, (7-hydroxycoumarin-4-yl)ethylglycine (HC), was site-specifically incorporated at the Phe69 site, close to the entrance of the selectivity filter of the NaK channel. Decreased fluorescence lifetime and elevated time-resolved anisotropy of NaK-F69HC in buffers with high K+/Na+ molar ratios indicated the K+ preference at the entrance of the NaK channel, consistent with previous crystal structure results of the NaK channel.