Miniaturized flow cytometer with 3D hydrodynamic particle focusing and integrated optical elements applying silicon photodiodes
Miniaturized flow cytometer with 3D hydrodynamic particle focusing and integrated optical elements applying silicon photodiodes
复制标题
DOI：
10.1007/s10404-010-0707-z
复制
发表时间：
2011-04-01
影响因子：
2.8
通讯作者：
Vellekoop, Michael
中科院分区：
文献类型：
--
作者：
Rosenauer, Michael;Buchegger, Wolfgang;Vellekoop, Michael
在这项研究中，设计，实现和测量结果的一种新的光流体系统能够进行基于吸光度的流式细胞仪分析。这个小型化的实验室平台，使用SU-8在硅衬底上制造，包括集成的聚合物基波导的光引导和双凹圆柱形透镜入射光聚焦。光学结构与微流体样品通道分离，导致光学灵敏度的显著增加。这允许应用标准固态激光器和通过锁定放大操作的标准硅基光电二极管，从而产生高度实用和有效的检测系统。易于制造的单层微流体结构使得能够独立地可调节的3D流体动力学样品聚焦到通道中的任意位置。为了确认流体动力学和光线跟踪模拟并表征系统，通过检测消光（轴向光损失）信号来研究用于活体染色的不同组的微粒和T淋巴细胞（Jurkat细胞系）。通过信号峰高/宽度的分析分类证明了这种紧凑型低成本/低功耗微流式细胞仪的高灵敏度和样品辨别能力。
In this study, the design, realization and measurement results of a novel optofluidic system capable of performing absorbance-based flow cytometric analysis is presented. This miniaturized laboratory platform, fabricated using SU-8 on a silicon substrate, comprises integrated polymer-based waveguides for light guiding and a biconcave cylindrical lens for incident light focusing. The optical structures are detached from the microfluidic sample channel resulting in a significant increase in optical sensitivity. This allows the application of standard solid-state laser and standard silicon-based photodiodes operated by lock-in-amplification resulting in a highly practical and effective detection system. The easy-to-fabricate single-layer microfluidic structure enables independently adjustable 3D hydrodynamic sample focusing to an arbitrary position in the channel. To confirm the fluid dynamics and raytracing simulations and to characterize the system, different sets of microparticles and T-lymphocyte cells (Jurkat cell line) for vital staining were investigated by detecting the extinction (axial light loss) signal. The analytical classification via signal peak height/width demonstrates the high sensitivity and sample discrimination capability of this compact low-cost/low-power microflow cytometer.