Magnetic resonance imaging as a surrogate measure for histological sub-chronic endpoint in a neonatal rat stroke model
Magnetic resonance imaging as a surrogate measure for histological sub-chronic endpoint in a neonatal rat stroke model
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DOI：
10.1016/j.brainres.2005.10.043
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发表时间：
2005-12-20
期刊：
影响因子：
2.9
通讯作者：
Vexler, ZS
中科院分区：
文献类型：
--
作者：
Derugin, N;Dingman, A;Vexler, ZS
简介：越来越多的人认识到，出生时CNS的不成熟会影响缺血性损伤和恢复，新生儿卒中的后果需要使用适合年龄的局灶性卒中模型进行研究。纳入磁共振成像（MRI）作为卒中进展的替代指标，在成人模型中提供了有用的信息，但新生儿卒中研究的益处尚未确定。研究方法：出生后7天（P7）的大鼠进行了3小时的短暂闭塞大脑中动脉（MCA），这是通过插入细丝通过颈外动脉或通过颈内动脉。MRI被用来描绘损伤的大小和模式急性，在大脑中动脉闭塞，7天后再灌注。结果如下：MCA闭塞期间弥散加权（DW）MRI可检测损伤的大小在两种手术后相似，并在所有动物中7天后导致组织学病变。如DW-和T2 W-MRI组合所示，各组内7天后个体动物的自发恢复程度显著不同，并通过相应的组织学证实。结论：MRI能够提供新生儿局灶性短暂性脑缺血后7天组织学结果大小的准确信息，这表明MRI可作为长期新生儿卒中研究中损伤进展的中间替代指标。(c)2005 Elsevier B. V.保留所有权利。
Introduction: It is becoming increasingly recognized that CNS immaturity at birth affects ischemic injury and recovery, and that the consequences of neonatal stroke need to be studied using age-appropriate focal stroke models. The inclusion of magnetic resonance imaging (MRI) as a surrogate measure of stroke progression has provided useful information in adult models, but the benefit for neonatal stroke studies is yet to be established. Methods: Postnatal 7-day (P7) rats were subjected to a 3-h transient occlusion of the middle cerebral artery (MCA) which was produced either by inserting a filament via the external carotid artery or via the internal carotid artery. MRI was used to delineate the size and pattern of injury acutely, during MCA occlusion, and 7 days following reperfusion. Results: The size of the diffusion-weighted (DW) MRI-detectable injury during MCA occlusion was similar following both surgical procedures and resulted in histological lesions 7 days later in all animals. The extent of spontaneous recovery in individual animals varied substantially 7 days later within each group, as was depicted by a combination of DW- and T2W-MRI and confirmed by the corresponding histology. Conclusions: The ability of MRI to provide accurate information on the size of histological outcome at 7 days after neonatal focal transient ischemia suggests that MRI is useful as an intermediate surrogate measure of injury progression in long-term neonatal stroke studies. (c) 2005 Elsevier B.V. All rights reserved.