Effects of iron limitation and aquatic humic substances on the growth of Microcystis aeruginosa
Effects of iron limitation and aquatic humic substances on the growth of Microcystis aeruginosa
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DOI：
10.1139/f99-131
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发表时间：
1999-10
影响因子：
2.4
通讯作者：
A. Imai;T. Fukushima;K. Matsushige
中科院分区：
文献类型：
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作者：
A. Imai;T. Fukushima;K. Matsushige
日本柏浦湖富营养化的水生腐殖质(AHSS)对铜绿微囊藻的生长有明显的抑制作用，其浓度与湖中的浓度相近。用XAD-8树脂分离和定量AHSS。这些分离的AHSS对铜绿假单胞菌培养物生长的影响在一种化学定义的培养基中进行了评估。还考察了铁限制条件下铜绿假单胞菌铁载体的产生情况。Kasumigaura湖AHSS中黄腐酸(AHSS的较低分子量组分)占AHSS的85%以上。在铁的限制下，铜绿假单胞菌产生了羟甲二酸类型的铁载体；然而，它们不能有效地实现实质性的恢复生长。当黄腐酸为2 mg干重·L-1时，即使产生铁载体，铜绿微囊藻的生长也受到显著抑制，与湖水中黄腐酸的浓度相当。生长抑制可能是由于铁引起的缺铁所致。
Aquatic humic substances (AHSs) from the eutrophic Lake Kasumigaura, Japan, significantly inhibited the growth of Microcystis aeruginosa at a concentration level similar to that found in the lake. AHSs were isolated and quantified using XAD-8 resin. The effects of these isolated AHSs on the growth of M. aeruginosa cultures were evaluated in a chemically defined medium. The production of siderophores, iron-specific complexing agents, of M. aeruginosa under iron limitation was also examined. Fulvic acid (the lower molecular size fraction of the AHSs) accounted for more than 85% of AHSs in Lake Kasumigaura. Under iron limitation, M. aeruginosa produced hydroxamate-type siderophores; however, they were not effective in enabling substantial recovery of growth. Microcystis aeruginosa growth was significantly inhibited when fulvic acid was 2 mg dry weight·L-1, similar to the concentration in the lake, even when siderophores were produced. Growth inhibition was probably due to a deficiency of iron caused by iron ...