Spermatogonia From Cryopreserved Testes of Critically Endangered Chinese Sturgeon Efficiently Colonized and Preferentially Proliferated in the Recipient Gonads of Yangtze Sturgeon.
Spermatogonia From Cryopreserved Testes of Critically Endangered Chinese Sturgeon Efficiently Colonized and Preferentially Proliferated in the Recipient Gonads of Yangtze Sturgeon.
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来自极度濒危中华鲟冷冻保存睾丸的精原细胞在长江鲟受体性腺中有效定殖并优先增殖。
DOI：
10.1007/s10126-022-10092-5
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发表时间：
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影响因子：
3
通讯作者：
Qiwei Wei
中科院分区：
文献类型：
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作者：
Huan Ye;Yutaka Takeuchi;Hao Du;Huamei Yue;Rui Ruan;Chuangju Li;Qiwei Wei
极度濒危的中华鲟，中华鲟，性成熟晚，体型大。生殖细胞移植是一种强有力的技术，用于在具有较小体型和较短世代时间的近亲受体中产生来自大体型物种的配子。为加速中华鲟繁殖，从3龄中华鲟精巢中采集供体精原细胞，经腹腔移植于孵化后7-8 d、世代间隔较短的长江鲟（Acipenserdabryanus）幼鱼体内。移植后2个月，供者精原细胞已在81.25%的受者性腺中定植，平均数量约为内源性原始生殖细胞的2倍。在接下来的2个月内，雌性的内源性生殖细胞分裂速率（2-3倍）比雄性的（1次）快，而定殖的供体来源的精原细胞在受体雌性和雄性中分别分裂约2-3倍和2次。此外，生殖细胞相关基因dazl、dead end和andvasa在移植鱼中的表达高于未移植鱼，表明供体精原细胞在受体中的整合和增殖。18 mpt时，供精原细胞在受体性腺中存活率为75.00%。这些结果表明，长江鲟性腺的体细胞微环境能够支持异种生殖细胞的长期定殖、增殖和存活。因此，本研究表明，小型长江鲟是中华鲟配子生产的理想受体。
The critically endangered Chinese sturgeon,Acipenser sinensis, presents late sexual maturity and has a large body size. Germ cell transplantation is a powerful technique for the production of gametes from large-bodied species in closely related recipients with a smaller body size and shorter generation time. To accelerate reproduction of Chinese sturgeon, donor spermatogonia collected from the cryopreserved testes of 3-year-old Chinese sturgeon were intraperitoneally transplanted into 7–8 days post-hatch larvae of Yangtze sturgeon (Acipenser dabryanus) with shorter generation interval. At 2 months post-transplantation (mpt), donor spermatogonia had colonized in the 81.25% of recipient gonads, with average numbers about two times those of endogenous primordial germ cells. Within the next 2 months, the rate of endogenous germ cell division in females (2–3 times) was faster than that in males (once), whereas colonized donor-derived spermatogonia divided about 2–3 times and twice in recipient females and males, respectively. Furthermore, the expression of germ cell-related genes,dazl,dead end, andvasa, in transplanted fish was higher than that in non-transplanted fish, suggesting the incorporation and proliferation donor spermatogonia in recipient. At 18 mpt, donor-derived spermatogonia survived in the 75.00% of recipient gonads. These results showed that the somatic microenvironment of Yangtze sturgeon gonad can support the long-term colonization, proliferation, and survival of xenogeneic germ cells. Thus, this study suggested that small-bodied Yangtze sturgeon is promising recipient as surrogate for Chinese sturgeon gamete production.