Identification of a new HLA-B allele (B*1576) by haplotype specific extraction
Identification of a new HLA-B allele (B*1576) by haplotype specific extraction
复制标题
DOI：
10.1016/j.humimm.2007.01.015
复制
发表时间：
2007-05-01
期刊：
影响因子：
2.7
通讯作者：
Albert, Ekkehard D.
中科院分区：
文献类型：
--
作者：
Dapprich, Johannes;Witter, Klaus;Albert, Ekkehard D.
通过序列特异性寡核苷酸探针（SSOP）和基于序列的分型（SBT）对潜在的骨髓供体进行常规分型，产生了不确定的亚型分型结果，提示了新的等位基因。一种基于磁珠的方法，单倍型特异性提取（HSE），用于分离二倍体样品成其单倍体组分。然后使用标准SBT对样本进行重新分型，显示新的人类白细胞抗原（HLA）等位基因，命名为B*1576。与标准SBT结合使用的HSE是一种方便和简单的工具，用于解决不明确的和新的等位基因组合，而不需要扩增或亚克隆。
Routine typing of a potential bone marrow donor by sequence-specific oligonucleotide probes (SSOP) and sequence based typing (SBT) produced inconclusive subtyping results, suggesting a new allele. A magnetic bead-based method, haplotype specific extraction (HSE), was used to separate the diploid sample into its haploid components. The sample was then re-typed using standard SBT, revealing a new human leukocyte antigen (HLA) allele, since named B*1576. HSE used in conjunction with standard SBT is a convenient and simple tool for resolving ambiguous and novel allele combinations without the need for amplification or subcloning.