A novel canine nuclear magnetic resonance spectroscopy-based metabolomics platform: Validation and sample handling
A novel canine nuclear magnetic resonance spectroscopy-based metabolomics platform: Validation and sample handling
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DOI：
10.1111/vcp.12954
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发表时间：
2021-08-24
影响因子：
1.2
通讯作者：
Lohi, Hannes
中科院分区：
文献类型：
--
作者：
Ottka, Claudia;Vapalahti, Katariina;Lohi, Hannes
背景代谢组学已被证明是一个宝贵的研究工具，提供全面的洞察全身代谢。然而，缺乏可扩展的和定量的方法与已知的参考区间（RI）和记录的重现性，阻止了代谢组学在临床环境中的使用。目的验证所开发的基于定量核磁共振（NMR）光谱的代谢组学平台对犬血清和血浆样品的分析能力，并确定最佳样品处理条件。方法采用Bruker公司的500 MHz核磁共振波谱仪对8247例犬标本进行分析。使用来自国际指南的统计方法，我们研究了方法的精密度，在各种长期和短期储存条件下的被测稳定性，以及与红细胞（RBC）长期接触的影响，以及采血管之间的差异。我们还筛选了对脂血症、溶血和胆红素血症的干扰。将结果与常规临床化学方法进行比较，并定义所有被测变量的RI。结果我们确定了123个被测对象的RI，其中大部分是以前未发表的。NMR平台结果的再现性总体上表现出色，并且可以通过遵循标准采血和处理指南来确保结果的完整性。结论由于定量结果、高重复性和可扩展性的优点，该犬代谢组学平台具有巨大的潜力，可用于许多临床和研究应用，以改善犬的健康和福祉。
Background Metabolomics has been proven to be an invaluable research tool by providing comprehensive insight into systemic metabolism. However, the lack of scalable and quantitative methods with known reference intervals (RIs) and documented reproducibility has prevented the use of metabolomics in the clinical setting. Objective The objective of this study was to validate the developed quantitative nuclear magnetic resonance (NMR) spectroscopy-based metabolomics platform for canine serum and plasma samples and determine optimal sample handling conditions for its use. Methods Altogether, 8247 canine samples were analyzed using a Bruker's 500 MHz NMR spectrometer. Using statistical approaches derived from international guidelines, we studied method precision, measurand stability in various long- and short-term storage conditions, as well as the effect of prolonged contact with red blood cells (RBCs), and differences among blood collection tubes. We also screened interferences with lipemia, hemolysis, and bilirubinemia. The results were compared against routine clinical chemistry methods, and RIs were defined for all measurands. Results We determined RIs for 123 measurands, most of which were previously unpublished. The reproducibility of the results of the NMR platform appeared generally outstanding, and the integrity of the results can be ensured by following standard blood drawing and processing guidelines. Conclusions Owing to the advantages of quantitative results, high reproducibility, and scalability, this canine metabolomics platform holds great potential for numerous clinical and research applications to improve canine health and well-being.