Expression and possible function of fibroblast growth factor 9 (FGF9) and its cognate receptors FGFR2 and FGFR3 in postnatal and adult retina
Expression and possible function of fibroblast growth factor 9 (FGF9) and its cognate receptors FGFR2 and FGFR3 in postnatal and adult retina
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DOI：
10.1002/jnr.20363
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发表时间：
2005-02-01
影响因子：
4.2
通讯作者：
Bugra, K
中科院分区：
文献类型：
--
作者：
Cinaroglu, A;Ozmen, Y;Bugra, K
成纤维细胞生长因子（FGF）是视网膜发育和存活的重要调节因子。我们检测了FGF 9及其首选受体FGFR 2 IIIc和FGFR 3 IIIc在该组织中的表达和分布。通过RT-PCR检测到整个大鼠视网膜中的FGF 9转录物，但在纯化的培养的Muller胶质细胞中不存在。转录本在北方印迹上显示为3.2-kb和4.0-kb条带，并且整个视网膜的Western印迹显示在30和55 kDa处的FGF 9免疫反应条带。FGF 9 mRNA表现出双相表达谱，在出生和成年时升高，但在终末视网膜分化（出生后4-14天）期间相对降低。抗体标记广泛地反映了这些发现：体内染色主要在视网膜内层（和成人的外网层）中观察到，而在培养的Muller神经胶质细胞中检测不到FGF 9。在成人中，FGF 9原位杂交也显示在视网膜内层可检测到信号。RT-PCR检测到FGFR 2 Ⅲ c和FGFR 3 Ⅲ c，Western blotting结果显示两种FGFR均以100 -200 kDa之间的多种形式存在。FGFR 2和FGFR 3抗体在视网膜内层，特别是在增殖培养的Muller神经胶质中显示出显著的标记。外源性FGF 9引起体外Muller胶质细胞增殖的剂量依赖性增加。这些数据表明FGF 9在视网膜分化和成熟中的作用，可能代表作用于神经胶质（和其他）细胞的神经源性因子。(C)2004威利-利斯公司
Fibroblast growth factors (FGFs) are important regulators of retinal development and survival. We examined the expression and distribution of FGF9 and its preferred receptors FGFR2IIIc and FGFR3IIIc in this tissue. FGF9 transcripts in whole rat retina were detected by RT-PCR but were not present in purified cultured Muller glia. Transcripts appeared as 3.2-kb and 4.0-kb bands on Northern blots, and Western blotting of whole retina revealed FGF9-immunoreactive bands at 30 and 55 kDa. FGF9 mRNA demonstrated a biphasic expression profile, elevated at birth and adulthood, but relatively decreased during terminal retinal differentiation (4-14 days postnatal). Antibody labeling broadly reflected these findings: staining in vivo was observed mainly in the inner retina (and outer plexiform layer in adults) whereas FGF9 was not detectable in cultured Muller glia. In adults, FGF9 in situ hybridization also showed a detectable signal in inner retina. FGFR2IIIc and FGFR3IIIc were detected by RT-PCR, and Western blotting showed both FGFRs existed as multiple forms between similar to100-200 kDa. FGFR2 and FGFR3 antibodies showed prominent labeling in the inner retina, especially in proliferating cultured Muller glia. Exogenous FGF9 elicited a dose-dependent increase in Muller glial proliferation in vitro. These data suggest a role for FGF9 in retinal differentiation and maturation, possibly representing a neuronally derived factor acting upon glial (and other) cells. (C) 2004 Wiley-Liss, Inc.