POLYPEPTIDE STRUCTURE OF TRANSMISSIBLE GASTROENTERITIS VIRUS
POLYPEPTIDE STRUCTURE OF TRANSMISSIBLE GASTROENTERITIS VIRUS
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DOI：
10.1099/0022-1317-29-1-25
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发表时间：
1975-01-01
影响因子：
3.8
通讯作者：
POCOCK, DH
中科院分区：
文献类型：
--
作者：
GARWES, DJ;POCOCK, DH
用聚丙烯酰胺凝胶电泳法对猪传染性胃肠炎冠状病毒纯化制剂的多肽进行了检测。四种主要多肽，VP1(摩尔.200000)、VP2(50000)、VP3(30000)和VP4(28500)以及VP1a(105000)和VP1b(80500)，其中VP1、VP3和VP4含有碳水化合物。用蛋白水解酶菠萝酶处理病毒粒子，可以去除表面突起和VP1，从而确定这种糖多肽是突起的主要结构成分。
The polypeptides of purified preparations of the coronavirus responsible for transmissible gastroenteritis of pigs have been examined by polyacrylamide gel electrophoresis. Four major polypeptides, VP1 (mol. wt. 200000), VP2 (50000), VP3 (30000) and VP4 (28500) and two minor polypeptides, VP1a (105000) and VP1b (80500) have been reproducibly demonstrated in the virion, of which VP1, VP3 and VP4 contain carbohydrate. Treatment of the virion with the proteolytic enzyme bromelain removes the surface projections and VP1, thus identifying this glycopolypeptide as the major structural component of the projection.