Water-based nanoparticulate polymeric system for protein delivery: permeability control and vaccine application.
Water-based nanoparticulate polymeric system for protein delivery: permeability control and vaccine application.
复制标题
用于蛋白质递送的水基纳米颗粒聚合物系统：渗透性控制和疫苗应用。
DOI：
10.1002/bit.1200
复制
发表时间：
2002
影响因子：
3.8
通讯作者：
Newman,MarkJ
中科院分区：
文献类型：
--
作者：
Prokop,Ales;Kozlov,Evgenii;Newman,GaleW;Newman,MarkJ
使用聚合物纳米颗粒作为药物载体的想法正在学术界和工业界受到越来越多的关注，纳米颗粒在蛋白质、药物和疫苗输送以及基因治疗应用中具有许多潜在的应用。在本文中，我们重点关注这种独特的药物递送技术，作为控制物质释放速率的方法，不仅用于蛋白质递送，还用于递送实验疫苗免疫原。纳米颗粒是根据水溶性聚合物之间的离子相互作用组装而成的，因此所得颗粒在生理介质中稳定。在用于组装纳米粒子的典型聚合物中，研究了不同的多糖、天然胺和聚胺。测试的包埋物质包括蛋白质和抗原。聚葡聚糖醛被纳入颗粒核心，以实现生理交联作为控制渗透性的方法。这导致了物质的长期保留，否则这些物质会迅速从纳米粒子中泄漏出来。交联实验的结果清楚地表明，根据交联程度，释放速率可以大大降低。对于疫苗抗原递送测试，我们测量了皮下和口服给药后的抗体产生。数据表明，当使用口服途径给药时，只有交联抗原具有免疫原性。本文中提供的数据主要讨论了纳米颗粒在口服递送疫苗抗原中的用途。 © 2002 Wiley periodicals, Inc. Biotechnol Bioeng 78: 459–466, 2002。
The idea of using polymeric nanoparticles as drug carriers is receiving an increasing amount of attention both in academia and industry, Nanoparticles have a number of potential applications in protein, drug and vaccine delivery, as well as gene therapy applications. In this article, we focus on this unique drug delivery technology as a method to control the release rate of substances, not only for protein delivery but also for delivering an experimental vaccine immunogen. Nanoparticles were assembled on the basis of ionic interaction between water‐soluble polymers so that the resulting particles were stable in physiologic media. Among the typical polymers used to assemble nanoparticles, different polysaccharides, natural amines, and poly‐amines were investigated. The entrapped substances tested included a protein and antigens. Polydextran aldehyde was incorporated into the particle core, to enable physiologic cross‐linking as a method to control permeability. This resulted in long‐term retention of substances that would otherwise rapidly leak out of the nanoparticles. Results of cross‐linking experiments clearly demonstrated that the release rate could be substantially reduced, depending on the degree of cross‐linking. For vaccine antigen delivery tests, we measured an antibody production after subcutaneous and oral administration. The data indicated that only the cross‐linked antigen was immunogenic when the oral route of administration was used. The data presented in this article address primarily the utility of nanoparticulates for oral delivery of vaccine antigen. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 459–466, 2002.