Site-selective RNA cleavage by DNA bearing a base pair-mimic nucleoside
Site-selective RNA cleavage by DNA bearing a base pair-mimic nucleoside
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DOI：
10.1021/ja045445s
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发表时间：
2005-01-19
影响因子：
15
通讯作者：
Sugimoto, N
中科院分区：
文献类型：
--
作者：
Nakano, S;Uotani, Y;Sugimoto, N
We have synthesized the deoxyadenosine derivative tethering a phenyl group (X), which mimics the Watson−Crick A/T base pair. The RNA/DNA hybrid duplexes containingXin the middle of the DNA sequence showed a similar thermal stability regardless of the ribonucleotide species (A, G, C, or U) opposite toX, probably because of the phenyl group stacking inside of the duplex accompanied by the opposite ribonucleotide base flipped in an extrahelical position. The RNA strand hybridized with the DNA strand bearingXwas cleaved on the 3‘-side of the ribonucleotide opposite toXin the presence of MgCl2, and the RNA sequence to be cleaved was not restricted. The site-specific RNA hydrolysis suggests that the DNA strand bearingXhas the advantage of the site-selective base flipping in the target sequence and the development of a “universal deoxyribozyme” to exclusively cleave a target RNA sequence.
