Glutathione S-transferases of human brain. Evidence for two immunologically distinct types of 26500-Mr subunits.
Glutathione S-transferases of human brain. Evidence for two immunologically distinct types of 26500-Mr subunits.
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人脑的谷胱甘肽S-转移酶。
DOI：
10.1042/bj2250375
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Awasthi,YC
中科院分区：
文献类型：
--
作者：
Theodore,C;Singh,SV;Hong,TD;Awasthi,YC
人脑含有一种阳离子型（pI8.3）和两种阴离子型（pI5.5和4.6）谷胱甘肽S-转移酶。阳离子形式（pI8.3）和阴离子较少的形式（pI5.5）不对应于先前在人体组织中表征的任何谷胱甘肽S-转移酶。这两种形式都是26500-Mr亚基的二聚体;然而，免疫学和催化特性表明这两种酶形式彼此不同。阳离子型（pI8.3）与抗人肝阳离子谷胱甘肽S-转移酶的抗体发生交叉反应，而阴离子型（pI5.5）则不发生交叉反应。此外，只有阳离子形式表达谷胱甘肽过氧化物酶活性。另一种阴离子形式（pI4.6）是24500-Mr和22500-Mr亚基的二聚体。双向凝胶电泳结果表明，人脑谷胱甘肽S-转移酶存在3种26500-Mr亚基，2种24500-Mr亚基和2种22500-Mr亚基。
Human brain contains one cationic (pI8.3) and two anionic (pI5.5 and 4.6) forms of glutathione S-transferase. The cationic form (pI8.3) and the less-anionic form (pI5.5) do not correspond to any of the glutathione S-transferases previously characterized in human tissues. Both of these forms are dimers of 26500-Mr subunits; however, immunological and catalytic properties indicate that these two enzyme forms are different from each other. The cationic form (pI8.3) cross-reacts with antibodies raised against cationic glutathione S-transferases of human liver, whereas the anionic form (pI5.5) does not. Additionally, only the cationic form expresses glutathione peroxidase activity. The other anionic form (pI4.6) is a dimer of 24500-Mr and 22500-Mr subunits. Two-dimensional gel electrophoresis demonstrates that there are three types of 26500-Mr subunits, two types of 24500-Mr subunits and two types of 22500-Mr subunits present in the glutathione S-transferases of human brain.