Proliferative state and radiosensitivity of human myeloma stem cells.
Proliferative state and radiosensitivity of human myeloma stem cells.
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人骨髓瘤干细胞的增殖状态和放射敏性。
DOI：
10.1038/bjc.1982.108
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发表时间：
1982-05
影响因子：
8.8
通讯作者：
Mizoguchi, H
中科院分区：
文献类型：
--
作者：
Shimizu, T;Motoji, T;Oshimi, K;Mizoguchi, H
人类骨髓瘤干细胞通过其在培养中形成菌落的能力来检测。从抽吸的骨髓中分离的细胞在半固体甲基纤维素中培养10天，培养基由植物血凝素刺激T淋巴细胞调节。形成的集落主要由淋巴浆细胞样细胞或浆细胞组成，患者骨髓瘤细胞中的免疫球蛋白也存在于集落细胞的细胞质中。菌落数量与细胞数和PHA-TCM浓度成正比。采用(3H)-dT-自杀技术研究多发性骨髓瘤（CFU-MM）增殖集落形成单位的比例，高特异性活性(3H)-dT杀死7例骨髓瘤患者中21-45%的CFU-MM。按单剂量co -y照射，2例再生损伤（Do）的平均剂量分别为1.00和1.63 Gy，外推数分别为1.6和2.0。
Human myeloma stem cells were detected by their capacity to form colonies in culture. Cells separated from aspirated marrow were cultured for 10 days in semi-solid methylcellulose with medium conditioned by T lymphocytes stimulated by phytohaemagglutinin (PHA-TCM). The colonies formed consisted mostly of lymphoplasmacytoid cells or plasma cells, and the immunoglobulins in the patients' myeloma cells were demonstrated also in the cytoplasm of the colony cells. The number of colonies were proportional to the number of cells plated and to the concentration of PHA-TCM. When the proportion of proliferating colony-forming units of multiple myeloma (CFU-MM) was studied using the (3H)-dT-suicide technique, the high-specific-activity (3H)-dT killed 21-45% of the CFU-MM in 7 myeloma patients. According to a single dose of Co-y-irradiation, the mean doses for impairment of regeneration (Do) were 1.00 and 1.63 Gy in 2 cases, the extrapolation numbers being 1.6 and 2.0.