Two developmentally distinct populations of neural crest cells contribute to the zebrafish heart.
Two developmentally distinct populations of neural crest cells contribute to the zebrafish heart.
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DOI：
10.1016/j.ydbio.2015.06.002
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发表时间：
2015-08-15
影响因子：
2.7
通讯作者：
Chen JN
中科院分区：
文献类型：
--
作者：
Cavanaugh AM;Huang J;Chen JN
心脏神经嵴细胞对于体外循环和肺循环系统分离的动物的流出道重构至关重要，但它们对单循环系统动物心脏发育的贡献尚不清楚。在这里，我们对神经嵴细胞进行了遗传标记，并研究了它们对斑马鱼心脏发育的贡献。我们鉴定了两种神经嵴细胞，它们在不同发育阶段对斑马鱼心血管系统的不同区室有贡献。在受精后24 - 30小时，神经嵴细胞流通过咽弓1和咽弓2进入原始心管的心肌，形成心肌细胞。发育3天后，第二波神经嵴细胞沿主动脉弓迁移，包裹腹主动脉内皮，并侵入动脉球。有趣的是，虽然FGF信号的抑制对神经嵴细胞与原始心管的整合没有影响，但它可以阻止这些细胞参与流出道，表明神经嵴细胞对FGF信号的不同反应。此外，斑马鱼神经嵴消融可导致多种心脏缺陷，包括心率降低、心肌成熟缺陷和无法从第二心野募集祖细胞。这些发现增加了我们对神经嵴细胞对心脏发育的贡献的理解，并为心脏成熟对这些细胞的需求提供了见解。
Cardiac neural crest cells are essential for outflow tract remodeling in animals with divided systemic and pulmonary circulatory systems, but their contributions to cardiac development in animals with a single-loop circulatory system are less clear. Here we genetically labeled neural crest cells and examined their contribution to the developing zebrafish heart. We identified two populations of neural crest cells that contribute to distinct compartments of zebrafish cardiovascular system at different developmental stages. A stream of neural crest cells migrating through pharyngeal arches 1 and 2 integrates into the myocardium of the primitive heart tube between 24 and 30 hours post fertilization and gives rise to cardiomyocytes. A second wave of neural crest cells migrating along aortic arch 6 envelops the endothelium of the ventral aorta and invades the bulbus arteriosus after three days of development. Interestingly, while inhibition of FGF signaling has no effect on the integration of neural crest cells to the primitive heart tube, it prevents these cells from contributing to the outflow tract, demonstrating disparate responses of neural crest cells to FGF signaling. Furthermore, neural crest ablation in zebrafish leads to multiple cardiac defects, including reduced heart rate, defective myocardial maturation and a failure to recruit progenitor cells from the second heart field. These findings add to our understanding of the contribution of neural crest cells to the developing heart and provide insights into the requirement for these cells in cardiac maturation.