Confocal reflection imaging of 3D fibrin polymers
Confocal reflection imaging of 3D fibrin polymers
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DOI：
10.1016/j.bcmd.2005.12.033
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发表时间：
2006-03-01
影响因子：
2.3
通讯作者：
Friedl, P
中科院分区：
文献类型：
--
作者：
Hartmann, A;Boukamp, P;Friedl, P
利用共聚焦反射显微镜重建细胞外基质（ECM）是一种监测ECM结构、细胞-基质相互作用以及ECM结构随时间变化的物理方法。我们在这里表明，共聚焦反射成像是有用的，在重建的纤维结构的3D纤维蛋白晶格和成纤维细胞嵌入其中的高分辨率唇250 nm。连同由成纤维细胞沉积的胶原蛋白的共聚焦荧光显微镜，该技术允许通过基质细胞监测纤维蛋白重塑。总之，共聚焦反射显微镜是一种实时监测纤维蛋白基质重塑的有价值的技术，例如在血栓形成、伤口愈合和肿瘤形成期间。(c)2006年爱思唯尔公司All rights reserved.
The reconstruction of extracellular matrix (ECM) by confocal reflection microscopy is a physical approach for monitoring the ECM structure, cell-matrix interactions and changes of E;CM structure over time. We here show that confocal reflection imaging is useful in reconstructing the fibrillar architecture of 3D fibrin lattices and fibroblasts embedded therein at high resolution Lip to 250 nm. Together with confocal fluorescence microscopy of collagen deposited by fibroblasts, this technique allows the monitoring of fibrin remodeling by stromal cells. In conclusion, confocal reflection microscopy is a valuable technique for real-time monitoring of the remodeling of fibrin matrices, Such as during thrombus formation, wound healing, and tumor formation. (c) 2006 Elsevier Inc. All rights reserved.