Antioxidant status of bilirubin and uric acid in patients diagnosed with Plasmodium falciparum malaria in Douala.
Antioxidant status of bilirubin and uric acid in patients diagnosed with Plasmodium falciparum malaria in Douala.
复制标题
DOI：
10.3923/pjbs.2008.1646.1649
复制
发表时间：
2008-06-15
期刊：
影响因子：
--
通讯作者：
Honore, Fotso Kuate
中科院分区：
文献类型：
--
作者：
Bertrand, Kouam Eric;Mathieu, Ndomou;Honore, Fotso Kuate
氧化应激和抗氧化剂状态的变化与疟疾的发病机制有关。为了评估与恶性疟疾感染相关的胆红素和尿酸的抗氧化水平，对喀麦隆杜阿拉的60名未经治疗的患者(30名男性和30名女性)进行了筛查。健康体检者65例(男29例，女36例)为对照组。用Jendrassik-Grof法计算总胆红素和结合胆红素，用Barham-Trin der法测定尿酸。结果表明，疟疾患者血清总胆红素和结合胆红素水平(10.722+/-4.043和3.627+/-1.571 mg L(-1))显著高于对照组(6.830+/-2.436和1.777+/-0.729 mg L(-1))，且随着寄生虫数量的增加，胆红素水平显著升高(p<；0.050)。疟疾患者尿酸水平(56.262+/-13.963 mg L(-1))明显高于对照组(49.838+/-15.419 mg L(-1))(P=0.021)。疟疾患者的尿酸、寄生虫计数、总胆红素和结合胆红素在性别上没有显著差异。寄生虫数与总胆红素(r=0.320，p<；0.050)、结合胆红素(r=0.477，p<；0.001)、尿酸(r=0.060，p>；0.050)、总胆红素(r=0.729，p<；0.001)呈正相关。从这项研究中推测，血浆胆红素和尿酸水平的升高可以对疟疾引起的氧化应激提供更多的保护。
Oxidative stress and changes in antioxidant status have been implicated in the pathogenesis of malaria. To assess the antioxidant level ofbilirubin and uric acid associated with falciparum malaria infection, 60 untreated patients (30 men and 30 women) in Douala, Cameroon were screened for the study. Sixty five healthy individuals (29 men and 36 women) were used as controls. Total and conjugated bilirubin were calculated using Jendrassik-Grof method while uric acid was determined using Barham-Trinder method. It was observed that total and conjugated bilirubins were significantly (p < 0.001) higher in malaria patients (10.722 +/- 4.043 and 3.627 +/- 1.571 mg L(-1), respectively) when compared to control (6.830 +/- 2.436 and 1.777 +/- 0.729 mg L(-1)) and these bilirubin levels increased significantly with parasite count (p < 0.050). There was also significant increased (p = 0.021) of uric acid in malaria patients (56.262 +/- 13.963 mg L(-1)) compared to controls (49.838 +/- 15.419 mg L(-1)). No significant differences based on sex were observed on uric acid, parasite count, total and conjugated bilirubins in malaria patients. Positive correlations were obtained between parasite count and total bilirubin (r = 0.320, p < 0.050), conjugated bilirubin (r = 0.477, p < 0.001), uric acid (r = 0.060, p > 0.050) and between total and conjugated bilirubin (r = 0.729, p < 0.001). From this study, it has been hypothesized that the augmentation of plasma level ofbilirubin and uric acid could provide more protection against oxidative stress induced by malaria.