Immunolocalization of apolipoprotein E in the testis and epididymis of the rat.
Immunolocalization of apolipoprotein E in the testis and epididymis of the rat.
复制标题
载脂蛋白 E 在大鼠睾丸和附睾中的免疫定位。
DOI：
10.1095/biolreprod50.3.535
复制
发表时间：
1994
影响因子：
3.6
通讯作者：
Schreiber,JR
中科院分区：
文献类型：
--
作者：
Olson,LM;Zhou,X;Schreiber,JR
载脂蛋白（apo）E是一种在几种脂蛋白表面发现的35 kDa蛋白质，已在许多外周组织中检测到，并被认为具有促进细胞间胆固醇/脂质转移的功能。我们检测了载脂蛋白E在幼年大鼠（21日龄）、青春期前大鼠（34-36日龄）和性成熟大鼠（75-80日龄）睾丸和附睾中的表达。通过多克隆兔抗大鼠载脂蛋白E抗体和标准免疫细胞化学技术定位载脂蛋白E。在所有大鼠的试验间质中均观察到apo E强阳性染色。通过在连续切片上使用3 β-羟基类固醇脱氢酶抗体将含Apo E的细胞鉴定为Leydig细胞。与性成熟大鼠相反，两组较年轻的大鼠在与Sertoli和生殖细胞相关的生精小管内也显示出apo E阳性染色。此外，在所有年龄大鼠的附睾头、体和尾的基质中观察到apo E强阳性染色。这项研究表明，载脂蛋白E是差异定位在开发过程中的测试，表明调节和/或胆固醇转运的作用。
Apolipoprotein (apo) E, a 35-kDa protein found on the surface of several lipoproteins, has been detected in many peripheral tissues and is postulated to function in facilitating the transfer of cholesterol/lipids between cells. We examined the expression of apo E in the testes and epididymides of juvenile rats (21 days old), prepubescent rats (34–36 days old) and sexually mature rats (75–80 days old). Apo E was localized by means of a polyclonal rabbit anti-rat apo E antibody and standard immunocytochemical techniques. Strong positive staining for apo E was observed in the interstitial space of the tests from all rats. Apo E-containing cells were identified as Leydig cells through use of a 3 β-hydroxysteroid dehydrogenase antibody on serial sections. In contrast to sexually mature rats, the two younger groups of rats also showed positive staining for apo E within the seminiferous tubule associated with Sertoli and germ cells. In addition, strong positive staining for apo E was observed in the stroma of the caput, corpus, and cauda epididymides from rats of all ages. This study demonstrates that apo E is differentially localized during development of the tests, suggesting a regulatory and/or cholesterol transport role.