Magnetic Field-Induced Alignment of Nanofibrous Supramolecular Membranes: A Molecular Design Approach to Create Tissue-like Biomaterials
Magnetic Field-Induced Alignment of Nanofibrous Supramolecular Membranes: A Molecular Design Approach to Create Tissue-like Biomaterials
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磁场诱导的纳米纤维超分子膜排列：一种创建组织样生物材料的分子设计方法
DOI：
10.1021/acsami.0c05191
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发表时间：
2020
影响因子：
9.5
通讯作者：
S. Azevedo, Helena
中科院分区：
文献类型：
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作者：
Radvar, Elham;Shi, Yejiao;Grasso, Salvatore;Edwards-Gayle, Charlotte J.;Liu, Xitong;Mauter, Meagan S.;Castelletto, Valeria;Hamley, Ian W.;Reece, Michael J.;S. Azevedo, Helena
报道了一种通过芳香族阳离子肽与透明质酸（HA）自组装和磁场下纳米纤维排列来制造纳米纤维膜的分子设计方法。肽被设计为在 C 末端含有由四个苯丙氨酸残基组成的块，通过疏水缔合和芳香堆积驱动其自组装，并具有赖氨酸残基的带正电结构域，用于与 HA 静电相互作用。这两个块通过具有可变数量的氨基酸并且能够采用不同构象的连接体连接。 Zeta 电位测量和圆二色性证实了它们的正电荷和可变构象（无规卷曲、β-折叠或 α-螺旋），这取决于 pH 值和序列。通过荧光光谱、小角 X 射线散射和透射电子显微镜检查，它们的自组装表明在微摩尔范围内形成了纤维状纳米结构。当肽与HA结合时，形成水凝胶或平板膜。由于苯丙氨酸残基的高抗磁各向异性，分子结构调节膜的机械行为，并且纳米纤维在磁场方向上排列。在磁性排列膜上培养的间充质干细胞沿纳米纤维的方向伸长，支持其在软组织工程中的应用。
A molecular design approach to fabricate nanofibrous membranes by self-assembly of aromatic cationic peptides with hyaluronic acid (HA) and nanofiber alignment under a magnetic field is reported. Peptides are designed to contain a block composed of four phenylalanine residues at the C-terminus, to drive their self-assembly by hydrophobic association and aromatic stacking, and have a positively charged domain of lysine residues for electrostatic interaction with HA. These two blocks are connected by a linker with a variable number of amino acids and the ability to adopt distinct conformations. Zeta potential measurements and circular dichroism confirm their positive charge and variable conformation (random coil, β-sheet, or α-helix), which depend on the pH and sequence. Their self-assembly, examined by fluorescence spectroscopy, small-angle X-ray scattering, and transmission electron microscopy, show the formation of fiberlike nanostructures in the micromolar range. When the peptides are combined with HA, hydrogels or flat membranes are formed. The molecular structure tunes the mechanical behavior of the membranes and the nanofibers align in the direction of magnetic field due to the high diamagnetic anisotropy of phenylalanine residues. Mesenchymal stem cells cultured on magnetically aligned membranes elongate in direction of the nanofibers supporting their application for soft tissue engineering.