Quantitative analysis of rat synaptosomes and cerebrum using high-resolution 1H magnetic resonance spectroscopy.
Quantitative analysis of rat synaptosomes and cerebrum using high-resolution 1H magnetic resonance spectroscopy.
复制标题
使用高分辨率 1H 磁共振波谱对大鼠突触体和大脑进行定量分析。
DOI：
10.1016/0009-8981(92)90014-h
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Prichard,JW
中科院分区：
文献类型：
--
作者：
Petroff,OA;Burlina,AP;Black,J;Prichard,JW
本研究探讨了1H磁共振波谱的实用性，以研究一个标准的突触体富集制备（P2小球）从大鼠大脑。该制剂含有高浓度的N-乙酰天冬氨酸和γ-氨基丁酸和低浓度的谷氨酰胺，表明它们实际上富含神经元胞质溶胶。突触体含有大脑一半的乳酸和谷氨酰胺。丙氨酸、天冬氨酸、谷氨酸和琥珀酸在突触体和大脑中的浓度相同。
This study explored the utility of1H magnetic resonance spectroscopy to study a standard synaptosomally enriched preparation (P2 pellet) made from rat cerebrum. The preparation contained high concentrations ofN-acetylaspartate and γ-aminobutyric acid and low concentrations of glutamine, indicating that they were in fact rich in neuronal cytosol. Synaptosomes contained half the lactate and glutamine of cerebrum. Alanine, aspartate, glutamate, and succinate had the same concentrations in synaptosomes and cerebrum.