Characterization of GhRac1 GTPase expressed in developing cotton (Gossypium hirsutum L.) fibers
Characterization of GhRac1 GTPase expressed in developing cotton (Gossypium hirsutum L.) fibers
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DOI：
10.1016/j.bbaexp.2004.06.008
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发表时间：
2004-09-17
期刊：
影响因子：
--
通讯作者：
Triplett, BA
中科院分区：
文献类型：
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作者：
Kim, HJ;Triplett, BA
细胞骨架的组装在决定棉纤维细胞长度和形态方面起着重要的作用，并受到发育的调节。与其他植物细胞一样，纤维中的细胞骨架组装是如何调节的尚不清楚。最近，拟南芥中的几种Rac/Rop GTP酶被证明通过控制细胞骨架的组装来调节根毛和花粉管的各向同性和极性细胞生长。GhRac 1是从棉籽中分离的Rac/Rop GTfamily的成员，在快速生长的棉花组织中大量表达。GhRac 1与拟南芥Rac/Rop基因组IV亚家族的序列相似性最高。GhRac 1在E.体外酶活性测定表明，大肠杆菌诱导产生功能性GT3。与棉纤维中发现的其他Rac/Rop GTP酶相比，GhRac 1在纤维发育的伸长阶段高度表达，当纤维伸长速率下降时，表达急剧下降。在最大棉纤维伸长的阶段，最高的GhRac 1表达的关联表明，GhRac 1的GTbind可能是一个潜在的调节纤维伸长通过控制细胞骨架组装。由爱思唯尔公司出版
Cytoskeleton assembly plays an important role in determining,cotton fiber cell length and morphology and is developmentally regulated. As in other plant cells, it is not clear how cytoskeletal assembly in fibers is regulated. Recently, several Rac/Rop GTPases in Arabidopsis were shown to regulate isotropic and polar cell growth of root hairs and pollen tubes by controlling assembly of the cytoskeleton. GhRac1, isolated from cottonseeds, is a member of the Rac/Rop GTPase family and is abundantly expressed in rapidly growing cotton tissues. GhRac1 shows the greatest sequence similarity to the group IV subfamily of Arabidopsis Rac/Rop genes. Overexpression of GhRac1 in E. coli led to the production of a functional GTPase as shown by in vitro enzyme activity assay. In contrast to other Rac/Rop GTPases found in cotton fiber, GhRac1 is highly expressed during the elongation stage of fiber development with expression decreasing dramatically when the rate of fiber elongation declines. The association of highest GhRac1 expression during stages of maximal cotton fiber elongation suggests that GhRac1 GTPase may be a potential regulator of fiber elongation by controlling cytoskeletal assembly. Published by Elsevier B.V.