Inhibition of Agrobacterium tumefaciens biofilm formation by acylase I-immobilized polymer surface grafting of a zwitterionic group-containing polymer brush
Inhibition of Agrobacterium tumefaciens biofilm formation by acylase I-immobilized polymer surface grafting of a zwitterionic group-containing polymer brush
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DOI：
10.1016/j.bej.2019.107372
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发表时间：
2019-12-15
影响因子：
3.9
通讯作者：
Terada, Akihiko
中科院分区：
文献类型：
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作者：
Bao, Qian;Xie, Li;Terada, Akihiko
生物膜是细菌细胞间通讯的热点，主要由N-酰基高丝氨酸内酯（AHLs）作为信号化合物介导。用于膜过滤的聚合物材料需要高透水性和生物膜抑制剂。因此，在本研究中，设计了一种亲水性和酶固定的聚合物膜，第一次，以防止生物膜的形成，通过降解AHLs。以聚乙烯膜为基材，采用辐射接枝聚合法（RIGP）接枝甲基丙烯酸缩水甘油酯（GMA）。GMA中的环氧基进一步转化为二甲基氨基-γ-丁酸（DMGABA）以增加亲水性。为了防止通过细胞-细胞通讯形成生物膜，将具有或不具有活性的群体猝灭酶酰化酶I固定到DMGABA膜片上，分别提供酶活性DMGABA（EI-DMGABA）和无活性DMGABA（Ina-EI-DMGABA）片。与GMA（84.3度）相比，DMGABA的引入改善了亲水性，静态接触角为30.2度。在流动池生物膜形成实验中，使用AHL进行群体感应的根癌农杆菌生物膜形成在EI-DMGABA片上被显著抑制，但在Ina-EI-DMGABA片上没有。证明活性AHL降解酶固定在亲水性聚合物表面上的概念的成功结果可能为应用于抑制界面上不利的生物膜形成铺平了道路。
Membrane processes for water purification and wastewater treatment are impacted by biofilm formation, a hotspot for bacterial cell-cell communication, mainly mediated by N-acyl homoserine lactones (AHLs) as signal compounds. Polymer materials for membrane filtration require high water permeability and biofilm deterrents. Therefore, in this study, a hydrophilic and enzyme-immobilizing polymer membrane was designed, for the first time, to prevent biofilm formation by degrading AHLs. A polyethylene membrane (PE) sheet was grafted as a substratum with glycidyl methacrylate (GMA) by radiation-induced graft polymerization (RIGP). The epoxy group in GMA was further converted into dimethylamino-gamma-butyric acid (DMGABA) to increase hydrophilicity. To prevent biofilm formation by cell-cell communication, the quorum-quenching enzyme, acylase I, with or without activity was immobilized onto a DMGABA membrane sheet, providing enzymatically active DMGABA (EI-DMGABA) and inactive DMGABA (Ina-EI-DMGABA) sheets, respectively. The introduction of DMGABA improved hydrophilicity with a static contact angle of 30.2 degrees, as compared to GMA (84.3 degrees). In a flow cell biofilm formation experiment, biofilm formation of Agrobacterium tumefaciens, using AHLs for quorum sensing, was dramatically inhibited on an EI-DMGABA sheet, but not on an Ina-EI-DMGABA sheet. The successful result to prove the concept of active AHL-degrading enzyme immobilized onto a hydrophilic polymer surface likely paves the way for the application to inhibition of unfavorable biofilm formation onto an interface.