Characterization of Enkephalin Release from Rat Striatum
Characterization of Enkephalin Release from Rat Striatum
复制标题
大鼠纹状体脑啡肽释放的表征
作者：
I. Lindberg;J. Dahl
摘要：使用蛋氨酸和亮氨酸脑啡肽特异性抗血清，我们研究了这些肽从大鼠纹状体切片中释放的特征。组织中只有 2-3% 的脑啡肽可以通过钾去极化释放；苍白球、丘脑和伏隔核也释放出类似的百分比。无法检测到海马体释放的脑啡肽。两种脑啡肽的纹状体释放同样受到灌注介质中钾和钙水平变化的影响。锂对基础或钾刺激释放没有影响； tyr-arg 不影响任一肽的基础释放。在这些实验中使用的短期潜伏期内，纹状体脑啡肽水平保持稳定。
Abstract: Using antisera specific for methionine‐ and leucine‐enkephalin, we studied the characteristics of the release of these peptides from rat striatal slices. Only 2–3% of the total tissue stores of enkephalin could be released by potassium depolarization; similar percentages were released from globus pallidus, thalamus, and nucleus accumbens. Enkephalin release from hippocampus could not be detected. The striatal release of both enkephalins was affected similarly by changes in potassium and calcium levels in the superfusion medium. Lithium has no effect on either basal or potassium‐stimulated release; tyr‐arg did not affect basal release of either peptide. Striatal enkephalin levels were stable during the short‐term incubation periods used in these experiments.