Estimated Drainage of Carbon from the Tricarboxylic Acid Cycle for Protein Synthesis in Suspension Cultures of Paul's Scarlet Rose Cells.
Estimated Drainage of Carbon from the Tricarboxylic Acid Cycle for Protein Synthesis in Suspension Cultures of Paul's Scarlet Rose Cells.
复制标题
保罗猩红玫瑰细胞悬浮培养物中用于蛋白质合成的三羧酸循环中碳的估计排出量。
DOI：
10.1104/pp.57.2.304
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发表时间：
1976
期刊：
影响因子：
7.4
通讯作者：
J. Fletcher
中科院分区：
文献类型：
--
作者：
L. Hunt;J. Fletcher
从三羧酸循环中排出的用于蛋白质合成的碳量（碳原子的摩尔数）与保罗猩红玫瑰悬浮培养物生长期间以2天为间隔从循环中释放的CO（2）的摩尔数进行了比较。我们的结论是，在蛋白质合成最快的时期（第0-4天），从三羧酸循环中排出的用于蛋白质合成的碳是以CO（2）形式释放的碳的六分之一。到第8天，以CO（2）形式释放的碳量的三十分之一被纳入蛋白质中。净蛋白质合成在第8天停止，但CO（2）/培养物的演变以其最大速率持续到第10天。基于向3天龄细胞（最大蛋白质合成年龄）提供标记底物3小时后蛋白质中的（14）C与CO（2）的回收率，计算出类似的比率。乙酸-1-（14）C和乙酸-2-（14）C的供应表明，八分之一至等量的碳被掺入蛋白质中，作为CO（2）释放。当提供（14）C-标记的三羧酸循环中间产物时，掺入蛋白质的（14）C与释放到CO（2）中的（14）C之比在1/0.9至1/4.9之间。从三羧酸循环中排出的用于蛋白质合成的碳量的保守估计是从该循环中以CO（2）形式释放的量的四分之一。
The amount of carbon (mumoles of carbon atoms) drained from the tricarboxylic acid cycle for protein synthesis was compared with mumoles of CO(2) released from the cycle at 2-day intervals during the growth of suspension cultures of Paul's Scarlet rose. We concluded that during the period of most rapid protein synthesis (day 0-4) one-sixth as much carbon was drained from the tricarboxylic acid cycle for protein synthesis as was released as CO(2). By day 8, one-thirtieth of the amount of carbon released as CO(2) was incorporated into protein. Net protein synthesis stopped on day 8, but the evolution of CO(2)/culture continued at its maximum rate until day 10.Similar ratios were calculated based on the recovery of (14)C in protein versus CO(2) following a 3-hr provision of labeled substrates to 3-day-old cells (age of maximum protein synthesis). Provision of acetate-1-(14)C and acetate-2-(14)C indicated from one-eighth to an equal amount of carbon was incorporated into protein as was released as CO(2). When (14)C-labeled intermediates of the tricarboxylic acid cycle were provided, the ratio of (14)C incorporated into protein versus that evolved in CO(2) ranged from 1/0.9 to 1/4.9.Following a critical analysis of the methods used, it was concluded that during periods of rapid protein synthesis, a conservative estimate of the amount of carbon drained from the tricarboxylic acid cycle for protein synthesis was one-fourth of the amount evolved as CO(2) from the cycle.