Optimization of a concentrated growth factor/mesoporous bioactive glass composite scaffold and its application in rabbit mandible defect regeneration
Optimization of a concentrated growth factor/mesoporous bioactive glass composite scaffold and its application in rabbit mandible defect regeneration
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DOI：
10.1039/d3bm00805c
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发表时间：
2023-08-08
影响因子：
6.6
通讯作者：
Meng，Lingqiang
中科院分区：
文献类型：
--
作者：
Ma，Mengran;Shen，Wenjing;Meng，Lingqiang
颌面部骨缺损的修复和再生在口腔医学领域仍然是一个巨大的挑战。然而，人工材料有限的骨诱导活性和昂贵的生物活性物质限制了其临床应用。本研究旨在构建一种经济高效的浓缩生长因子/介孔生物活性玻璃(CGF/MBG)复合支架用于骨再生。系统比较了不同形态CGFS的生化组成和生物学效应，结果表明CGF条件培养液有效地促进了同种异体BMSCs的增殖、迁移和成骨。凝胶相CGF(GpCGF)比液态CGF(LpCGF)和液/凝胶混合相CGF(LgpCGF)具有更好的生物活性和骨诱导活性，并被进一步应用于CGF/MBG支架的构建。体外研究表明，与gpCGF条件培养液共培养进一步增强了MBG的生物相容性，增加了细胞在支架上的黏附和增殖。在此基础上，采用纤维蛋白凝胶形成法(CGF/FG/MBG)和冷冻干燥法(fdCGF/MBG)两种复合方法构建支架，并比较了CGFS的体内生物学效应。在兔下颌骨缺损模型中，CGF/FG/MBG复合支架较fdCGF/MBG具有更高的成骨效率。综上所述，CGF/FG/MBG复合支架有望成为临床移植的一种有效的骨修复方法，而基于gpCGF和纤维蛋白凝胶形成方法的CGF复合支架有望提高现有临床骨修复材料的生物活性和骨诱导活性，为未来骨科生物材料的发展提供了新的思路。
Maxillofacial bone defect repair and regeneration remains a tremendous challenge in the field of stomatology. However, the limited osteoinductivity of artificial materials and the high cost of bioactive agents restrain their clinical translation. This study aimed to construct an economical and efficient concentrated growth factor/mesoporous bioactive glass (CGF/MBG) composite scaffold for bone regeneration. The biochemical composition and biological effects of different forms of CGFs were systematically compared, and the results showed that CGF-conditioned medium effectively promoted proliferation, migration and osteogenesis of allogenic BMSCs. Gel phase CGF (gpCGF) exhibited superior bioactivity and osteoinductivity to liquid phase CGF (lpCGF) and liquid/gel mixed phase CGF (lgpCGF), and was further applied to construct CGF/MBG scaffolds. In vitro studies demonstrated that co-culture with gpCGF-conditioned medium further enhanced the biocompatibility of MBG, increasing cell adhesion and proliferation on the scaffold. On this basis, two compositing approaches to construct the scaffold by fibrin gel formation (CGF/FG/MBG) and freeze-drying (fdCGF/MBG) were applied, and the biological efficacy of CGFs was compared in vivo. In a rabbit mandibular defect model, higher osteogenic efficiency in in situ bone regeneration of CGF/FG/MBG composite scaffolds was proved, compared with fdCGF/MBG. Taken together, the CGF/FG/MBG composite scaffold is expected to be an efficient bone repairing therapy for clinical translation, and the CGF-composited scaffold using gpCGF and the fibrin gel formation method is a promising way to enhance the bioactivity and osteoinductivity of current clinical bone repairing materials, providing new thoughts on the development of future orthopedic biomaterials.