MAPPING OF THE FOCAL ADHESION KINASE (FADK) GENE TO MOUSE CHROMOSOME-15 AND HUMAN-CHROMOSOME-8
MAPPING OF THE FOCAL ADHESION KINASE (FADK) GENE TO MOUSE CHROMOSOME-15 AND HUMAN-CHROMOSOME-8
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DOI：
10.1007/bf00303256
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发表时间：
1995-02-01
期刊：
影响因子：
2.5
通讯作者：
KAY, ES
中科院分区：
文献类型：
--
作者：
FIEDOREK, FT;KAY, ES
粘着斑激酶（Focal adhesion kinase，pp 125 FAK或FAK）是一种细胞质蛋白酪氨酸激酶，在细胞与细胞外基质成分的相互作用以及暴露于多种激动剂（包括神经肽）时受到刺激。FAK缺乏Src同源性SH 2和SH 3结构域，在物种间高度保守，并且可能重新呈现参与新信号转导途径的酪氨酸激酶家族的原型。我们已经确定了小鼠黏着斑激酶基因3'非翻译区的序列变异，并使用基于PCR的寡核苷酸杂交分析将小鼠基因（Fadk）定位到骨髓细胞瘤病原癌基因（Myc）远端的15号染色体（Chr）。在一组体细胞杂交细胞系中，人同源物（PTK 2）已被分配给人Chr 8。基于小鼠和人染色体图谱的同线性，人PTK 2基因的位置可能对应于人Chr 8 q24-qter。
Focal adhesion kinase (pp125FAK or FAK) is a cytoplasmic protein-tyrosine kinase stimulated in response to cell interactions with extracellular matrix components and by exposure to a variety of agonists, including neuropeptides. FAK lacks Src-homology SH2 and SH3 domains, is highly conserved across species, and may re-present the prototype for a tyrosine kinase family involved in novel signal transduction pathways. We have identified sequence variants in the 3' untranslated regions of the focal adhesion kinase gene in mice and used a PCR-based oligonucleotide hybridization assay to map the mouse gene (Fadk) to Chromosome (Chr) 15 distal to the myelocytomatosis protooncogene (Myc). The human homolog (PTK2) has been assigned to human Chr 8 on a panel of somatic hybrid cell lines. On the basis of synteny of mouse and human chromosomal maps, the position of the human PTK2 gene probably corresponds to human Chr 8q24-qter.