Mechanisms of lysosomal enzyme release from human polymorphonuclear leukocytes. Effects of phorbol myristate acetate.
Mechanisms of lysosomal enzyme release from human polymorphonuclear leukocytes. Effects of phorbol myristate acetate.
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DOI：
10.1083/jcb.66.3.647
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发表时间：
1975-09
期刊：
影响因子：
--
通讯作者：
Weissmann G
中科院分区：
文献类型：
--
作者：
Goldstein IM;Hoffstein ST;Weissmann G
PMA增强了嗜天青颗粒酶、β-葡萄糖醛酸苷酶以及溶菌酶从细胞松弛素B处理的暴露于酵母多糖颗粒或C5 a的PMN的释放。PMA在纳摩尔浓度下是有活性的，对细胞没有毒性，并且在细胞暴露于刺激物之前存在短暂的持续时间（0-1分钟）时是最有效的。β-葡萄糖醛酸酶没有释放的显着量从PMN的暴露于PMA单独，在没有刺激，如酵母聚糖或C5 a。相反，只有特定的颗粒酶，溶菌酶，从未受刺激的细胞释放。暴露于PMA的细胞的电子显微照片显示，与对照细胞相比，可见的细胞质微管的数量增加。PMA对溶酶体酶（β-葡萄糖醛酸酶）释放的增强似乎与对特定颗粒酶（溶菌酶）释放的影响无关，而很可能是由于PMA诱导的细胞环鸟苷酸升高。
PMA enhanced release of the azurophil granule enzyme, beta- glucuronidase, as well as lysozyme, from cytochalasin B-treated PMN's exposed to either zymosan particles or C5a. PMA was active at nanomolar concentrations, was not toxic to the cells, and was most effective when present for brief durations (0-1 min) before exposure of the cells to the stimuli. Beta-glucuronidase was not released in significant amounts from PMN's exposed to PMA alone, in the absence of stimuli such as zymosan or C5a. In contrast, only the specific granule enzyme, lysozyme, was released from unstimulated cells. Electron micrographs of cells exposed to PMA revealed an increase in the number of visible cytoplasmic microtubules as compared to control cells. Enhancement of lysosomal enzyme (beta-glucuronidase) release by PMA appears to be independent of effects on release of specific granule enzymes (lysozyme), but rather is likely due to PMA-induced elevations of cellular cGMP.